Benani 2009 J Neurosci Methods

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Benani A, Barquissau V, Carneiro L, Salin B, Colombani A-L, Leloup C, Casteilla L, Rigoulet M, Pénicaud L (2009) Method for functional study of mitochondria in rat hypothalamus. J Neurosci Methods 178:301-7.

» PMID: 19150628

Benani A, Barquissau V, Carneiro L, Salin B, Colombani A-L, Leloup C, Casteilla L, Rigoulet M, Penicaud L (2009) J Neurosci Methods

Abstract: Different roles of mitochondria in brain function according to brain area are now clearly emerging. Unfortunately, no technique is yet described to investigate mitochondria function in specific brain area. In this article, we provide a complete description of a procedure to analyze the mitochondrial function in rat brain biopsies. Our two-step method consists in a saponin permeabilization of fresh brain tissues in combination with high-resolution respirometry to acquire the integrated respiratory rate of the biopsy. In the first part, we carefully checked the mitochondria integrity after permeabilization, defined experimental conditions to determine the respiratory control ratio (RCR), and tested the reproducibility of this technique. In the second part, we applied our method to test its sensitivity. As a result, this method was sensitive enough to reveal region specificity of mitochondrial respiration within the brain. Moreover, we detected physiopathological modulation of the mitochondrial function in the hypothalamus. Thus this new technique that takes all cell types into account, and does not discard or select any mitochondria sub-population is very suitable to analyze the integrated mitochondrial respiration of brain biopsies.

Keywords: Brain, Hypothalamus, Mitochondria, Respiration Metabolism, Oxidative phosphorylation

O2k-Network Lab: FR Toulouse Casteilla L, FR Dijon Leloup C


Labels: MiParea: Respiration, Instruments;methods 


Organism: Rat  Tissue;cell: Nervous system  Preparation: Permeabilized tissue 

Regulation: ADP, Inhibitor, Substrate 


HRR: Oxygraph-2k