Chakraborty 2014 J Cardiovasc Dis

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Chakraborty M, Phillips ARJ, Macdonald J, Windsor JA, Hickey AJR (2014) Mitochondrial respiration in mononuclear cells and heart fibers in spontaneously hypertensive rats. J Cardiovasc Dis ISSN:2330-459.

» vol2-no1-3

Chakraborty M, Phillips ARJ, Macdonald J, Windsor JA, Hickey AJR (2014) J Cardiovasc Dis

Abstract: Mitochondrial function is altered in organs in hypertension and may be implicated in subclinical organ damage. Therefore measuring mitochondrial function may be clinically useful but, a biopsy of organ/tissue is required to measure mitochondrial function and that is invasive in the clinical setting. The aim of this study was to measure mitochondrial function from easily obtainable circulating mononuclear cells (MC) and the heart tissue of spontaneous hypertensive rats (SHR) at an early hypertensive and a compensated hypertrophic stage. Six and 14 months age SHR and Wistar control rats were divided into four groups and mitochondrial function was measured in MC and permeabilized heart fibers. MC showed decreased respiratory control ratios and decreased electron transfer-pathway capacity attributable to Complex I in SHR. These findings suggest that MC were less efficient at producing adenine triphosphate (ATP) and were less dependent on CI substrates. In contrast, heart tissue from SHR and Wistars showed similar mitochondrial function at both ages. Altered mitochondrial function was present in MC at a stage in the disease when it was not detectable in the heart. This study provides a protocol to study mitochondrial function from MC which may be correlated with subclinical organ damage in patients with hypertension.

Keywords: Hypertension, Mitochondria, Oxidative phosphorylation, Electron transport system, Subclinical organ damage, Spontaneous hypertensive rat, Mononuclear cells

O2k-Network Lab: NZ Auckland Hickey AJ


Labels: MiParea: Respiration, Instruments;methods  Pathology: Cardiovascular 

Organism: Rat  Tissue;cell: Heart, Blood cells  Preparation: Permeabilized cells, Permeabilized tissue 


Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, S, Gp, CIV, NS, ROX  HRR: Oxygraph-2k 


Correction

An Oroboros Oxygraph-2k was used in this publication, whereas the Anton Paar/Oroboros Oxygraph was the first-generation instrument for high-resolution respirometry, which was replaced by the Oxygraph-2k in 2002.