Devaux 2015 Abstract MiP2015

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Mitochondrial complexes I and II behave differently in two anoxia-tolerant species: adaptive mitochondrial plasticity may be related to the ability to undergo metabolic depression.


Renshaw G, Hickey A, Devaux J (2015)

Event: MiP2015

An adequate oxygen supply is crucial for vertebrate survival because ATP is almost exclusively produced by mitochondria though oxidative phosphorlyaltion (OXPHOS). Anoxia leads to ATP depletion and altered metabolic pathways including succinate accumulation which during re-oxygenation triggers a reverse of electron flow to CI accompanied by an enhanced deleterious reactive oxygen species (ROS) production [1]. A few species are able to survive prolonged periods of hypoxia and anoxia at tropical temperatures [2,3]. Two closely related elasmobranchs have very different adative responses to anoxia [2] while both can survive prolonged periods of anoxia the epaulette shark enters a phase of metabolic depression in response to hypoxia or anoxia while the grey carpet shark maintains its metabolic rate but releases additional red blood cells to prolong survival. Mitochondria in heart fibers from the anoxia tolerant epaulette shark maintained mitochondrial efficiency with a low ROS release in response to oxygen limitation, including anoxia [4]. The extent of mitochondrial plasticity in response to diminished oxygen (hypoxia or anoxia) and the triggers involved in this adative process are currently being investigated in our laboratories.

Our aim was to determine the response of mitochondrial complexes to elevated succinate after a bout of diminished oxygen in two closely related anoxia tolerant species. Sharks were acclimated in aerated sea-water (100L tanks) held at 22°C and were not fed 24 hours prior to sampling. Sharks were euthanised, the cerebellum isolated and homogenised in cold MiR05. Mitochondrial respiration was measured using high resolution respirometry (Oroboros Oxygraph-2k) with a SUIT protocol. Respiration flux (pmol O2 s-1 mg-1) was determined using DatLab 6.0 and statistical analysis were performed using SPSS™. Calculations were made to determine CI capacity, CII activation and coupling efficiency with and without a 20 min episode of anoxia followed by reoxygenation and stepwise succinate titrations (using a TIP2K microPump).

O2k-Network Lab: NZ Auckland Hickey AJ

Labels: MiParea: Respiration, Comparative MiP;environmental MiP 

Stress:Ischemia-reperfusion, Oxidative stress;RONS  Organism: Fishes  Tissue;cell: Nervous system  Preparation: Homogenate 

Regulation: Substrate  Coupling state: LEAK, OXPHOS, ET  Pathway: N, S  HRR: Oxygraph-2k, TIP2k  Event: D1, Poster, P-flash  MiP2015 


1-Hypoxia Ischemia Research Unit, School of Allied Health Science, Griffith Univ, Australia; 2-School Biol Sc, Univ Auckland; New Zealand. -

Abstract continued

CI OXPHOS was examined under i) normoxia; ii) hypoxia (PO2 = 1%); and iii) after 20 min of anoxia (followed by reoxygenation). Respiration rates were normalised to the respective normoxic CI OXPHOS for each species in order to estimate the potential loss of CI capacity. Exposure to hypoxia or anoxia/reoxygenation induced a decrease of CI capacity in both species. Post-anoxia, the CI capacity of mitochondria from the grey carpet shark was significantly less than those of the epaulette shark. Furthermore, the coupling efficiency of mitochondria from the epaulette shark increased significantly after anoxia/reoxygenation compared to those from the grey carpet shark. After 20 min of anoxia followed by 5 minutes of reoxgyenation, the CII substrate succinate was titrated from physiological concentrations (0-2 mM) until OXPHOSmax was reached. Succinate-stimulated mitochondrial respiration was normalised to the ET-pathway max measured for each species. Analysis revealed that succinate stimulated CII respiration following 20 min of anoxia compared to CII respiration in normoxia (controls) was significantly lower in the epaulette shark, reaching a 60% decrease in CII respiration at low levels of succinate (0.5 mM). In contrast, in the grey carpet shark the succinate stimulated CII respiration following 20 min of anoxia compared was significantly higher than the CII respiration in their controls, reaching a 40% increase at 2.5 mM.

Taken together these data indicate that the ES but not the GCS was able to maintain coupling efficiency and CI capacity even after 20 mins of anoxia followed by reperfusion. The ES but not the GCS responded to succinate accumulation by significantly decreasing CII respiration. Moreover these data suggest that the adaptive plasticity of ES mitochondria would support entry into metabolic depression as a protective response to anoxia/reoxygenation.

Figure 1

MiP2015 Devaux Figure.JPG

References and acknowledgements

  1. Chouchani ET, Pell VR, Gaude E, Aksentijević D, Sundier SY, Robb EL, Logan A, Nadtochiy SM, Ord EN, Smith AC, Eyassu F, Shirley R, Hu CH, Dare AJ, James AM, Rogatti S, Hartley RC, Eaton S, Costa AS, Brookes PS, Davidson SM, Duchen MR, Saeb-Parsy K, Shattock MJ, Robinson AJ, Work LM, Frezza C, Krieg T, Murphy MP (2014) Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS. Nature 515:431-5
  2. Chapman C, Renshaw GMC (2009) Haematological responses of the Grey Carpet Shark (Chiloscyllium punctatum) and the Epaulette Shark (Hemiscyllium ocellatum) to Anoxia and Re-oxygenation Exposure. Exp Zool Part A 311:422-38.
  3. Renshaw GMC, Kerrisk CB, Nilsson GE (2002) The role of adenosine in the anoxic survival of the epaulette shark, Hemiscyllium ocellatum. Comp Biochem Physiol B 131:133-41.
  4. Hickey AJR, Renshaw GMC, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: Depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182:91-100.