Drahota 2002 J Bioenerg Biomembr

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Drahota Z, Chowdhury SK, Floryk D, Mrácek T, Wilhelm J, Rauchova H, Lenaz G, Houstek J (2002) Glycerophosphate-dependent hydrogen peroxide production by brown adipose tissue mitochondria and its activation by ferricyanide. J Bioenerg Biomembr 34:105-13.

» PMID: 12018887

Drahota Z, Chowdhury SK, Floryk D, Mracek T, Wilhelm J, Rauchova H, Lenaz G, Houstek J (2002) J Bioenerg Biomembr

Abstract: Oxidation of glycerophosphate (GP) by brown adipose tissue mitochondria in the presence of antimycin A was found to be accompanied by significant production of hydrogen peroxide. GP-dependent hydrogen peroxide production could be detected by p-hydroxyphenylacetate fluorescence changes or as an antimycin A-insensitive oxygen consumption. One-electron acceptor, potassium ferricyanide, highly stimulated the rate of GP-dependent antimycin A-insensitive oxygen uptake, which was prevented by inhibitors of mitochondrial GP dehydrogenase (mGPDH) or by coenzyme Q(CoQ). GP-dependent ferricyanide-induced peroxide production was also determined luminometrically, using mitochondria or partially purified mGPDH. Ferricyanide-induced peroxide production was negligible, when succinate or NADH was used as a substrate. These results indicate that hydrogen peroxide is produced directly by mGPDH and reflect the differences in the transport of reducing equivalents from mGPDH and succinate dehydrogenase to the CoQ pool. The data suggest that more intensive production of reactive oxygen species may be present in mammalian cells with active mGPDH.

Keywords: Brown adipose tissue, Mitochondrial glycerophosphate dehydrogenase, Ferricyanide, Hydrogen peroxide

O2k-Network Lab: CZ Prague Houstek J, CZ Hradec Kralove Cervinkova Z, CA Winnipeg Fernyhough P, IT Bologna Lenaz G, IT Bologna Genova ML


Labels: MiParea: Respiration 

Stress:Oxidative stress;RONS 

Tissue;cell: Fat  Preparation: Isolated mitochondria 



HRR: Oxygraph-2k