Drahota 2005 Physiol Res

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Publications in the MiPMap
Drahota Z, Krivakova P, Cervinkova Z, Kmonickova E, Lotkova H, Kucera O, Houstek J (2005) Tert-butyl hydroperoxide selectively inhibits mitochondrial respiratory-chain enzymes in isolated rat hepatocytes. Physiol Res 54:67-72.

» PMID: 15717843 Open Access

Drahota Z, Krivakova P, Cervinkova Z, Kmonickova E, Lotkova H, Kucera O, Houstek J (2005) Physiol Res

Abstract: Sensitivity of various mitochondrial enzymes to oxidative damage was tested on isolated rat liver hepatocytes permeabilized by digitonin. In permeabilized hepatocytes normal respiratory control values were obtained and mitochondrial membranes remained intact. Respiratory rates of NADH-dependent (glutamate+malate, palmitylcarnitine + malate) and flavoprotein-dependent (succinate) substrates were determined in hepatocytes exposed for 5 min to 0.5-3 mM tert-butyl hydroperoxide before addition of digitonin. Our data showed that oxidation of NADH-dependent substrates is much more sensitive to oxidative stress than oxidation of flavoprotein-dependent ones, evidently due to the modification of iron-sulfur clusters or SH groups in the NADH dehydrogenase enzyme complex (Complex I).

Keywords: Hepatocytes, Mitochondrial enzymes Tert-butyl hydroperoxide

O2k-Network Lab: CZ_Prague_Houstek J, CZ_Hradec Kralove_Cervinkova Z


Labels: MiParea: Respiration 


Organism: Rat  Tissue;cell: Liver  Preparation: Permeabilized cells  Enzyme: TCA cycle and matrix dehydrogenases 

Coupling state: OXPHOS  Pathway:HRR: Oxygraph-2k