Gnaiger 2018 EBEC2018
The protonmotive force under pressure: an isomorphic analysis. |
Link: EBEC2018
Gnaiger E (2018)
Event: EBEC2018 Budapest HU
‘.. the sum of the electrical pressure difference and the osmotic pressure difference (i.e. the electrochemical potential difference) of protons’ [1] links to non-ohmic flux-force relationships between proton leak and protonmotive force (pmf). This is experimentally established, has direct consequences on mitochondrial physiology, but is theoretically little understood [2,3]. Here I distinguish pressure from potential differences (diffusion: Δμ_{H}+ or Δ_{d}F_{H}+; electric: ΔΨ or Δ_{el}F), to explain non-ohmic flux-force relationships on the basis of four thermodynamic theorems. (1) Einstein’s diffusion equation [4] explains the concentration gradient (dc/dz) in Fick’s law as the product of chemical potential gradient (the vector force and resistance determine the velocity, v, of a particle) and local concentration, c. This yields the chemical pressure gradient (van’t Hoff): d_{d}Π/dz = RT∙dc/dz. Flux [5] is the product of v and c; c varies with force. Therefore, flux-force relationships are non-linear. (2) The pmf is not a vector force; the gradient is replaced by a pressure difference, and local concentration by a distribution function or free activity, α. Flux is a function of α and force, J_{d} = b∙α∙Δ_{d}F_{B} = -b∙Δ_{d}Π_{B} [6]. (3) At Δ_{el}F = -Δ_{d}F_{H}+, the diffusion pressure of protons, Δ_{d}Π_{H}+ = RT∙Δ_{c}_{H}+ [Pa=J∙m^{-3}] is balanced by electric pressure, maintained by counterions of H^{+}. Diffusional and electric pressures are isomorphic, additive, and yield protonmotive pressure (pmp). (4) The dependence of proton leak on pmf varies with Δ_{el}F versus Δ_{d}F_{H}+, in agreement with experimental evidence. The flux-force relationship is concave at high mitochondrial volume fractions, but near-exponential at small mt-matrix volume ratios. Linear flux-pmp relationships imply a near-exponential dependence of the proton leak on the pmf.
• Bioblast editor: Gnaiger E
• O2k-Network Lab: AT Innsbruck Gnaiger E
Affiliations
- D. Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck
- Oroboros Instruments
- Innsbruck, Austria. - erich.gnaiger@oroboros.at
References
- Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Glynn Research, Bodmin. Biochim Biophys Acta Bioenergetics 1807:1507-38. - »Bioblast link«
- Garlid KD, Beavis AD, Ratkje SK (1989) On the nature of ion leaks in energy-transducing membranes. Biochim Biophys Acta 976:109-20. - »Bioblast link«
- Beard DA (2005) A biophysical model of the mitochondrial respiratory system and oxidative phosphorylation. PLOS Comput Biol 1(4):e36. - »Bioblast link«
- Einstein A (1905) Über die von der molekularkinetischen Theorie der Wärme geforderte Bewegung von in ruhenden Flüssigkeiten suspendierten Teilchen. Ann Physik 4, XVII:549-60. - »Bioblast link«
- Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65:1983-2002. - »Bioblast link«
- Gnaiger E (1989) Mitochondrial respiratory control: energetics, kinetics and efficiency. In: Energy transformations in cells and organisms. Wieser W, Gnaiger E (eds), Thieme, Stuttgart:6-17. - »Bioblast link«
Labels: MiParea: Respiration
Regulation: Flux control, Ion;substrate transport, mt-Membrane potential
Coupling state: LEAK
Event: Oral
MitoEAGLE