Huete-Ortega 2018 MiPschool Tromso E2

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Maria Huete-Ortega
High-resolution respirometry: new perspectives for the study of bioenergetics in algae and plants.

Link: MitoEAGLE

Huete-Ortega M, Komlodi T, Gnaiger E (2018)

Event: MiPschool Tromso-Bergen 2018

COST Action MitoEAGLE

Bioenergetics is the study of energy transformations in cells, mitochondria, and chloroplasts as a basis to perform biochemical work. In chloroplasts solar energy is acquired and assimilated through photosynthesis to synthesise the organic matter required for growth, which is associated with production of oxygen, while mitochondrial respiration consumes oxygen to transform organic matter into the chemical energy that fuels cellular activity. Energetic coupling between chloroplasts and mitochondria has been described. For example, mitochondrial physiology is involved in mitigating light stress in the photosynthetic pathway or chloroplast-generated reducing equivalents replace photophosphorylation as a source of ATP in the mitochondria. Therefore, a good functionality and cross-talk between both organelles is necessary to maintain metabolic integrity in plant cells. High-resolution respirometry (HRR) is widely used to assess mitochondrial respiration and other bioenergetic parameters of isolated mitochondria, cultured cells, tissue preparations and human biopsies. In the biomedical field of mitochondrial physiology and its clinical applications, studies with HRR relate to degenerative diseases and life style-linked preventive medicine. My current research aims to extend the experimental options of the Oroboros O2k developed by Oroboros Instruments for HRR to the study of mitochondrial respiration, photosynthesis and other bioenergetic parameters in algae and plants, including the development of new technology such as using a three-electrode system [1,2] inserted into the Oroboros Q2k to evaluate the redox state of the Coenzyme Q.


Bioblast editor: Huete-Ortega M, Plangger M O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck Oroboros


Affiliations

Huete-Ortega M(1), Komlodi T(1), Gnaiger E(1,2)
  1. Oroboros Instruments, Innsbruck
  2. Daniel Swarovski Research Lab, Mitochondrial Physiology, Dept Visceral, Transplant Thoracic Surgery Medical Univ Innsbruck; Austria. - maria.huete-ortega@oroboros.at

References

  1. Rich PR (1988) Patent of Q-electrode. European Patent no.85900699.1
  2. Moore AL, Dry IB, Wiskich JT (1988) Measurement of the redox state of the ubiquinone pool in plant mitochondria. FEBS 235:76-80.


Labels: MiParea: Respiration, Instruments;methods 


Organism: Plants 




HRR: Oxygraph-2k, O2k-Fluorometer  Event: E2