Iglesias-Gonzalez 2017 MITOEAGLE Obergurgl

From Bioblast
Jump to: navigation, search
Javier Iglesias-Gonzalez
Oscillations in mitochondrial ROS production as a new mechanism for cell cycle control.

Link: MitoEAGLE

Iglesias-Gonzalez J, Thomson C, Ishibashi S, Amaya E (2017)

Event: MitoEAGLE Obergurgl 2017

COST Action MitoEAGLE

Cell cycle presents synonymous mechanisms with positive feedbacks in order to provide a robust regulation during the processes of proliferation and differentiation [1], both essential for an appropriate development of the embryo. Mitochondria are the powerhouses of the cell but they are also involved in other processes such as cellular signaling and calcium buffering. However, the roles of mtROS during early vertebrate development has remained largely unknown. We have recently shown using Xenopus frog embryos with the genetically encoded ROS indicator HyPer that mtROS is increased after the fertilization and oscillates during each cell division. This process proves essential for the regulation by oxidation of key proteins such as the Cdc25c phosphatase. The changes in ROS levels are correlated with the oscillation in ATP levels, which's source is OXPHOS or glycolysis depending on the phase of the cell cycle. In order to target the source of mtROS in the electron transfer-pathway we performed and studied in a cell-free system (i.e. egg extract) combining high-resolution respirometry, hydrogen peroxide production and membrane potential [2]. Our study reveals the flavin adenine dinucleotide cofactor at the succinate dehydrogenase as the major source of mtROS, depending on the availability of substrates and in the pre-activation or not of the egg extract by calcium. Our results suggest a new mechanism to regulate the cell cycle mediated by the mitochondria that could open new opportunities in the field of the regenerative biology.

Keywords: Frog Bioblast editor: Kandolf G O2k-Network Lab: UK Manchester Galli GL


Labels: MiParea: Respiration, mt-Medicine 

Stress:Oxidative stress;RONS 




HRR: Oxygraph-2k  Event: A1, Oral 


Affiliations

Div Cell Matrix Biol & Regenerative Medicine, School Biological Sciences, Fac Biol, Medicine Health, Univ Manchester, UK. - Javier.Iglesias-Gonzalez@manchester.ac.uk

References

  1. Pomerening JR (2009) Positive feedback loops in cell cycle progression. FEBS Lett 583:3388-96.
  2. Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) Biomolecules 5:1319-88.