Irving 2017 MiP2017
Event: MiP2017ROUTINE respiration. We then added pyruvate and malate (2PM; exogenous substrate) and digitionin (0.8 uL per millions cells) to measure LEAK respiration. Subsequently, we sequentially added 10 uL ADP (3D), cytochrome c (3Dc), and glutamate (4G) to measure N-linked oxidative capacity (OXPHOS). We then added succinate (5S) to measures NS-linked OXPHOS, followed by CCCP titration (6U) to measure ET capacity, and antimycin A (7Ama) to measure residual oxygen consumption (ROX). Overall, there was a significant increase in ROUTINE respiration following the acute bout of exercise (4.3±0. 4 vs. 3.8±0.4 pmols/sec/million, p<0.05). There were no differences in LEAK, N-linked OXPHOS, NS-linked OXPHOS, or ET-pathway (p>0.05). In conclusion, ROUTINE respiration in freshly isolated PBMC cells is elevated in college swimmers in response to an acute bout of exercise. Finally, we will also discuss some methodological consideration with regards to sample preparation.
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Tissue;cell: Blood cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: N, NS, ROX HRR: Oxygraph-2k
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