Koopman 2017 MiPschool Obergurgl

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Werner Koopman
Mitochondrial function and dysfunction: ROS detection and redox analysis. Koopman_Presenation

Link: MitoEAGLE

Koopman WJH (2017)

Event: MiPschool Obergurgl 2017

COST Action MitoEAGLE

Mitochondria are considered as a relevant source of reactive oxygen species (ROS), especially under pathological conditions. During the last decade, a large collection of chemical and genetically-encoded fluorescent sensors have been developed for the measurement of ROS levels, ROS production and redox homeostasis. In this presentation, various aspects of these sensors will be discussed using examples from the literature and the author’s own research [1,2,3,4,5,6].


Bioblast editor: Kandolf G O2k-Network Lab: NL Nijmegen Koopman WJ


Labels: MiParea: Instruments;methods 

Stress:Oxidative stress;RONS 





Event: E2, Review 


Affiliations

Dept Biochem (286), Radboud Univ Medical Center, Nijmegen, The Netherlands.- w.koopman@ncmls.ru.nl

References

  1. Basit F, van Oppen LMPE, Schöckel L, Bossenbroek HM, van Emst-de Vries SE, Hermeling JCW, Grefte S, Kopitz C, Heroult M, Willems PHGM, Koopman WJH (2017) Mitochondrial complex I inhibition triggers a mitophagy-dependent ROS increase leading to necroptosis and ferroptosis in melanoma cells. Cell Death Dis 8:e2716.
  2. Sieprath T, Corne TDJ, Willems PHGM, Koopman WJH, De Vos WH (2016) Integrated high-content quantification of intracellular ROS levels and mitochondrial morphofunction. Adv Anat Embryol Cell Biol 219:149-77.
  3. Oparka M, Walczak J, Malinska D, van Oppen L, Szczepanowska J, Koopman WJH, Wieckowski MR (2016) Quantifying ROS levels using CM-H2DCFDA and HyPer. Methods 109:3-11.
  4. Willems PHGM, Rossignol R, Dieteren CEJ, Murphy MP, Koopman WJH (2015) Redox homeostasis and mitochondrial dynamics. Cell Metab 22:207-18.
  5. Forkink M, Willems PHGM, Koopman WJH, Grefte S (2015) Chapter 15: Reactive oxygen species quantification using hydroethidium. Meth Mol Biol 1264:161-9.
  6. Willems PHGM, Wanschers B, Esseling J, Szklarzyk R, Kudla U, Duarte I, Nooteboom M, Forkink M, Swarts H, Gloerich J, Nijtmans LJ, Koopman WJH, Huynen M (2013) BOLA1 is an aerobic protein that prevents mitochondrial morphology changes induced by glutathione depletion. Antioxid Redox Signal 18:129-38.