Mracek T

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COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


Mracek T

MitoPedia topics: EAGLE 

COST: Member


Name Mracek Tomas, RNDr.

Head of department

Mracek Tomas.jpg
Deptartment of Bioenergetics,

Institute of Physiology,

Czech Academy of Sciences, CZ

Address Videnska 1083, 142 20
City Prague
Country Czech Republic
O2k-Network Lab CZ Prague Houstek J



Gnaiger 2019 MitoFit Preprint Arch2019Gnaiger E, Aasander Frostner E, Abdul Karim N, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v4.
Pecinova 2017 Oxid Med Cell Longev2017Pecinova A, Drahota Z, Kovalcikova J, Kovarova N, Pecina P, Alan L, Zima M, Houstek J, Mracek T (2017) Pleiotropic effects of biguanides on mitochondrial reactive oxygen species production. Oxid Med Cell Longev 7038603.
Pravenec 2017 Physiol Res2017Pravenec M1, Zídek V, Landa V, Mlejnek P, Šilhavý J, Šimáková M, Trnovská J, Škop V, Marková I, Malínská H, Hüttl M, Kazdová L, Bardová K, Tauchmannová K, Vrbacký M, Nůsková H, Mráček T, Kopecký J, Houštěk J (2017) Mutant Wars2 gene in spontaneously hypertensive rats impairs brown adipose tissue function and predisposes to visceral obesity. Physiol Res 66:917-24.
Pravenec 2016 Physiol Genomics2016Pravenec M, Mlejnek P, Zídek V, Landa V, Šimáková M, Šilhavý J, Strnad H, Eigner S, Eigner Henke K, Škop V, Malínská H, Trnovská J, Kazdová L, Drahota Z, Mráček T, Houštěk J (2016) Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue. Physiol Genomics 48:420-7.
Hartmannova 2016 Hum Mol Genet2016Hartmannová H, Piherová L, Tauchmannová K, Kidd K, Acott PD, Crocker JF, Oussedik Y, Mallet M, Hodaňová K, Stránecký V, Přistoupilová A, Barešová V, Jedličková I, Živná M, Sovová J, Hůlková H, Robins V, Vrbacký M, Pecina P, Kaplanová V, Houštěk J, Mráček T, Thibeault Y, Bleyer AJ, Kmoch S (2016) Acadian variant of Fanconi syndrome is caused by mitochondrial respiratory chain complex I deficiency due to a non-coding mutation in complex I assembly factor NDUFAF6. Hum Mol Genet 25:4062-79.
Melenovsky 2016 Eur J Heart Fail2016Melenovsky V, Petrak J, Mracek T, Benes J, Borlaug BA, Nuskova H, Pluhacek T, Spatenka J, Kovalcikova J, Drahota Z, Kautzner J, Pirk J, Houstek J (2016) Myocardial iron content and mitochondrial function in human heart failure: a direct tissue analysis. Eur J Heart Fail 19:522-30.
Hejzlarova 2015 Biochem J2015Hejzlarová K, Kaplanová V, Nůsková H, Kovářová N, Ješina P, Drahota Z, Mráček T, Seneca S, Houštěk J (2015) Alteration of structure and function of ATP synthase and cytochrome c oxidase by lack of Fo-a and Cox3 subunits caused by mitochondrial DNA 9205delTA mutation. Biochem J 466:601-11.
Kluckova 2015 Cell Death Dis2015Kluckova K, Sticha M, Cerny J, Mracek T, Dong L, Drahota Z, Gottlieb E, Neuzil J, Rohlena J (2015) Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation. Cell Death Dis 6:e1749.
Pecina 2014 Biochim Biophys Acta Clinical2014Pecina P, Houstkova H, Mracek T, Pecinova A, Nuskova H.Tesarova M, Hansikova H, Janota J, Zemanc J, Houstek J (2014) Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry. Biochim Biophys Acta Clinical 2:62–71.
Rauchova 2011 Horm Metab Res2010Rauchová H, Mrácek T, Novák P, Vokurková M, Soukup T (2010) Glycerol-3-phosphate dehydrogenase expression and oxygen consumption in liver mitochondria of female and male rats with chronic alteration of thyroid status. Horm Metab Res 43:43-7.
Vrbacky 2007 Biochim Biophys Acta2007Vrbacky M, Drahota Z, Mracek T, Vojtískova A, Jesina P, Stopka P, Houstek J (2007) Respiratory chain components involved in the glycerophosphate dehydrogenase-dependent ROS production by brown adipose tissue mitochondria. Biochim Biophys Acta 1767:989-97.
Honzik 2006 Placenta2006Honzik T, Drahota Z, Bohm M, Jesina P, Mracek T, Paul J, Zeman J, Houstek J (2006) Specific properties of heavy fraction of mitochondria from human-term placenta - Glycerophosphate-dependent hydrogen peroxide production. Placenta 27:348-56.
Drahota 2002 J Bioenerg Biomembr2002Drahota Z, Chowdhury SK, Floryk D, Mrácek T, Wilhelm J, Rauchova H, Lenaz G, Houstek J (2002) Glycerophosphate-dependent hydrogen peroxide production by brown adipose tissue mitochondria and its activation by ferricyanide. J Bioenerg Biomembr 34:105-13.


Alan 2017 MiP20172017
Alan Lukas
Role of MLQ protein in the structure and function of mammalian F1FO ATP-synthase.
Pecina 2017 MiP20172017
Pecina Petr
Mitochondrial ATP synthase disorders investigated by quantitative proteomics of CRISPR-Cas9 knockout cell lines.
Brazdova 2017 MiP20172017
Brazdova Andrea
Pleiotropic effects of biguanides on mitochondrial reactive oxygen species production.
Pecinova 2017 MiP20172017
Pecinova A
Targeting tumor cell proliferation by inhibition of mitochondrial metabolic pathways.
Cunatova 2017 MiP20172017
Cunatova Kristyna
COX4-1/4-2 knock-out causes total cytochrome c oxidase deficiency and partial impairment of mitochondrial proteosynthesis.
Mracek 2017 MiP20172017
Mracek Tomas
Knockout of DAPIT, an accessory subunit of mitochondrial ATP synthase, causes right ventricular hypertrophy and pulmonary hypertension.
Efimova 2017 MiP20172017
Efimova Iulija
Purification and subunit composition analysis in the models of mammalian ATP synthase deficiencies.
Kovalcikova 2017 MiP20172017
Kovalcikova Jana
Mitochondrial protein TMEM70: key role in the biogenesis of ATP synthase verified in a mouse knockout model.
Pajuelo-Reguera 2017 MiP20172017
David Pajuelo-Reguera
COX4-1/COX4-2 cell knock-out/knock-in models for the study of cytochrome c oxidase regulation.
Tauchmannova 2015 Abstract MiP20152015Assembly of subunit Fo-a into mammalian ATP synthase.
Nuskova 2015 Abstract MiPschool London 20152015Insufficient energy provision or increased oxidative stress – what matters more in ATP synthase deficiencies?
Kovarova 2015 Abstract MiP20152015Tissue- and species-specific differences in cytochrome c oxidase assembly induced by SURF1 defects.
Rohlena 2015 Abstract MiP20152015Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation.
Karbanova 2015 Abstract MiP20152015CD36 does not directly participate in mitochondrial fatty acid transport and oxidation.
Mracek 2015 Abstract MiP20152015Myocardial iron and mitochondrial function in failing and non-failing human heart: direct tissue analysis.
Pecinova 2015 Abstract MiP20152015Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue.
Kovalcikova 2015 Abstract MiP20152015Derangements of myocardial mitochondrial function in patients with end-stage heart failure is associated with reduced endonuclease G.
Pecina 2015 Abstract MiP20152015Pharmacological inhibition of fatty-acid oxidation synergistically enhances the effect of L-asparaginase in childhood ALL cells.
Mracek 2014 Abstract MiP20142014Function of mitochondrial energy provision apparatus is compromised in patients with chronic heart failure. Mitochondr Physiol Network 19.13.
Houstek 2014 Abstract MiP20142014Alteration of structure and function of ATP synthase and cytochrome c oxidase by lack of Fo-a and Cox3 subunits caused by mitochondrial DNA 9205delTA mutation.
Pecina 2014 Abstract MiP20142014Manifestation of mitochondrial disorders of nuclear origin in lymphocytes.
Pecina 2013 Abstract MiP20132013Pecina P, Houšťková H, Mráček T, Pecinová A, Nůsková H, Tesařová M, Hansíková H, Janota J, Zeman J, Houštěk J (2013) The use of lymphocytes for diagnostics of mitochondrial oxidative phosphorylation disorders. Mitochondr Physiol Network 18.08.


        • Benefits for COST and for the COST Action MitoEAGLE
Thinking about our potential involvement, I would propose following:
WG3 - we can easily squeeze here with comparison of Oroboros and Seahorse, which we try to do anyway in order to establish the value of Seahorse. Here, we can join N. Larssons group, if it joins mtEAGLE (did not find them here yet).
WG3/WG1 – Measurement of lymphocytes and using lymphocytes for cross-laboratory validation –as we now have quite extensive experience with lymphoid cells, we can participate in protocol development and so on. We are also happy to host some visiting researchers and teach them our expertise.
WG1 – we have just about started screening of mito phenotypes in rat RI strains (SHRxBN). In the end, data will be available in database, but most likely as some ratios of various respiratory states to make them usable for QTL mapping. We can deposit original data into mitoEAGLE database as well. It will be more genotype x phenotype study than age, gender etc. x phenotype and it would include rats, which would require broadening the mitoEAGLE scope to laboratory rodents in general and not to keep it focussed on mice only.
If there would be any chance to utilise “Short-Term Scientific Missions” to send young team members to other participating labs and learn some new techniques (just looking at the participant list, e.g. fluorescent measuring of ATP in the lab of C. Chinopoulos springs into mind), it would be great added value of this COST project for us. And of course, we are also happy to host others in our lab, as I proposed with lymphocyte programme (and it does not have to be limited to it).

Participated at