Murray 2018 MiPschool Tromso E1

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Andrew Murray
OXPHOS protocols: understanding the patterns.

Link: MitoEAGLE

Murray AJ (2018)

Event: MiPschool Tromso-Bergen 2018

COST Action MitoEAGLE

For the mitochondrial physiologist, the substrate-uncoupler-inhibitor titration (SUIT) protocol represents a flexible method for the assessment of several facets of mitochondrial respiratory function, often within a single protocol. For example, protocols can provide information on absolute and relative capacity for the oxidation of particular substrates (e.g. fatty acid derivatives) or the function of the electron transfer system (ETS) and its associated pathways. SUIT protocols can also address questions relating to coupling efficiency and ideally include quality control steps to validate the integrity of a mitochondrial preparation. It is vital to select the most appropriate protocol when designing experiments, with this determined by the scientific question as well as the tissue studied and mitochondrial preparation used. In this session, I will discuss issues to be aware of when selecting an OXPHOS protocol and interpreting the results. We will touch on complementary analytical techniques that can aid interpretation of SUIT data, and I will open a discussion on some current issues relating to protocol design. During the session, we will refer to the MitoPedia SUIT library [1], a recent innovation by Oroboros Instruments to support users, with the opportunity to provide feedback on this developing resource.


Bioblast editor: Beno M, Plangger M


Affiliations

Dept Physiology, Development Neuroscience, Univ Cambridge, UK

References

  1. MitoPedia: SUIT


Labels: MiParea: Instruments;methods, mt-Awareness 






Event: E1