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Sample - DatLab 7 |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
In the window Sample, information is entered and displayed for the sample (Sample type, Cohort, Sample code, Sample number, Subsample number and sample concentration). Entries can be edited any time during the experiment in real-time or during post-experiment analysis. All related results are recalculated instantaneously with the new parameters. Initially, the Edit experiment window displays information from the last file recorded and saved while connected to the O2k.
Abbreviation: F3
- Experimental code Up to 10 digits. The File search function (Ctrl+F) lists all files with identical experimental code within a selected directory.
- File recorded by (read only) shows the user who recorded the file. While connected to the O2k, the User code can be changed by left clicking "Change user".
- O2k-serial number (read only) automatically recorded.
- Power O2k (read only) as defined in Oroboros O2k\ O2k configuration window in the upper menu.
- Chamber The following entries are entered separately for the left (A) and right (B) O2k-chamber.
- Protocol Enter the protocol name.
- Sample type/ Cohort/ Sample code/ Sample number Enter information about sample used in each chamber. No sample is added in O2 calibration experiments or upon cleaning.
- Unit Select a unit to express the concentration or amount of sample in the HRR assay.
- Million cells - cell count.
- mg - mg of protein, wet mass or dry mass.
- Unit - units of another marker of sample size.
- Unit Select a unit to express the concentration or amount of sample in the HRR assay.
- Concentration Enter the sample concentration (e.g. Million cells/mL, mg Wm/mL, mg mt-protein/mL). The corresponding amount of sample is calculated based on the O2k-chamber volume.
- Amount Alternatively, enter the sample amount (e.g. biopsy Wm) if a known amount of sample is added into the chamber. The corresponding sample concentration is calculated based on the chamber volume.
- Medium Name of the incubation medium in the O2k-chamber.
- Chamber volume The default is 2.00 mL. For further calculations of oxygen flux, H2O2 flux, etc., it is important to define the effective volume of the O2k-chamber actually used.
- Data recording interval [s] (read only) is selected in the O2k control window or press F7.
- Comments For display additional information about the experiment and printing in the Experimental log window.
- Reset to system default Left click to reset values to system default.
- Cancel Left click to proceed quickly with the experiment, and edit any time later.
Previous DatLab versions
- Up to DatLab 6:
- The window name was Edit experiment.
- Up to DatLab 6:
MitoPedia O2k and high-resolution respirometry: DatLab
