Short-Term Scientific Missions MitoEAGLE

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MiPsociety
COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


 

Short-Term Scientific Missions MitoEAGLE


COST Action MitoEAGLE

Grant periods

GP GP time span MC Meeting Work and Budget Plan STSM application deadline 1st Call STSM application deadline 2nd Call STSM time span STSM final report deadline
2016-09-12 Brussels, BE 2016-09-12
1 2016-11-01 to 2017-04-30 Barcelona, ES 2017-03-21 WBP 1 2016-11-01 2016-11-20 to 2017-03-25 2017-04-25
2 2017-05-01 to 2018-04-30 Hradec Králové, CZ 2017-11-15 WBP 2 2017-05-13 2017-09-28 2017-06-01 to 2017-10-31

2017-11-01 to 2018-03-25

2018-04-25
3 2018-05-01 to 2019-04-30 Jurmala, LV 2018-09-18 WBP3.1/WBP3.2 - 2019-02-28 2018-05-15 to 2019-03-25

2019-04-25

4 2019-05-01 to 2020-04-30 Belgrade, RS 2018-10-13 2019-05-01 to 2020-03-25 2020-04-25
5 2020-05-01 to 2020-09-11 2020-05-01 to 2020-08-01 2020-09-01

STSM Grant Period 3

Awardees 2018 May 15th to 2019 March 25th
  • The final dates of the STSMs will have to be negotiated between the applicant and the host.
34,650.- EUR (Work and Budget Plan)
50% will be allocated to Inclusiveness Target Countries
MC decision on 2017-11-18: STSM should be more focused to target specific goals in MoU, devoted to complete specific WG tasks towards a manuscript
  • 1st Call: Deadline 2019-02-28
STSM applicant Country Host Project WG
Asander Frostner Eleonor Aasander Frostner Eleonor Sweden Gnaiger E (AT) MitoEAGLE proficiency training WG1 WG4
Vlad Avram Avram Vlad Florian Romania Elmer E (SE) MitoEAGLE WG4 manuscript WG4
Calabria Elisa Calabria Elisa Italy Elmer E (SE) WG4 Blood cells workshop and retreat: interlaboratory manuscript and data organization. WG4
Calisto Filipa Portugal McMillan D (NL) Proton translocation mechanism of Alternative Complex III WG1
Gama Pérez Pau Spain Larsen S (DK) WG1 and 2 human skeletal muscle workshop WG2
Garcia Souza LF Garcia-Souza Luiz F Austria Elmer E (SE) Comparison of platelets isolation methods on respiration and activation markers WG4
Karavaeva Iuliuia Karavaeva Iuliia Denmark Gnaiger E (AT) Evaluation of mitochondrial respiration of brown adipocytes using non-canonical respiratory substrates for deorphanizing mitochondrial metabolite transporters. WG3
Maseko Tumisang Edward Czech Republic Muntane J Study of mitochondrial dysfunction induced by Sorafenib in primary hepatocytes and hepatocyte-derived cell line (HepG2), WG3 and WG4 WG3, WG4
Siewiera Karolina Siewiera Karolina Poland Elmer E (SE) WG 4 blood cell workshop and retreat WG4
Silaidos Carmina Silaidos Carmina Germany Elmer E (SE) Working Group 4 blood cell workshop WG4
Vilks Karlis Latvia Rustan AC (NO) Examination of molecular mechanisms of acylcarnitine pathophysiology WG1
Zdrazilova Lucie Zdrazilova Lucie Czech Republic Gnaiger E (AT) Mitochondrial respiration in different cell lines measured in the 2.0 mL and 0.5 mL chambers of the O2k-FluoRespirometer (Oroboros): an experimental basis for platform comparison with the Seahorse XFe24 Bioanalyzer (Agilent) WG1
Vendelin Marko Vendelin Marko Estonia Schlattner U (FR) Collaboration within MitoEAGLE network WG1
Gonzalez-Franquesa Alba Denmark Garcia-Roves PM (ES) White adipose tissue mitochondrial respiration protocols - Experiments and Results discussion WG1, WG2
Silva Filomena Pereira da Silva Grilo da Silva Filomena Portugal Gnaiger E (AT) Unspecific effect of etomoxir on mitochondrial respiration WG1, WG3
Giakoumaki Ifigeneia United Kingdom Wuest RC (NL) Use of high-resolution respirometry to assess skeletal muscle mitochondrial respiration in humans after a 60-day bed rest WG2

STSM Grant Period 2

Awardees 2017 June 01st to 2017 October 31st and 2017 Novemver 01st to 2018 March 25th
  • The final dates of the STSMs will have to be negotiated between the applicant and the host.
35,000.- EUR (Work and Budget Plan)
4,800.- EUR (addition from rest budget of the MC-Meeting in Hradec Kralvoe)
  • 1st Call: Deadline 2017-05-13
  • 2nd Call: Deadline 2017-10-03
STSM applicant Country Host Project WG
Chinopoulos Christos

Chinopoulos Christos

Hungary Gnaiger E (AT) Simultaneous measurements of oxygen, mitochondrial membrane potential and NADH using the O2k-NextGen WG1
Danila Maria-Daniela Romania Labieniec-Watala M (PL) CA15203 MitoEAGLE WG4
Dalmao Fernandez Andrea Spain Rustan AC (NO) Fatty acid and glucose metabolism in cybrids from osteoarthritis subjects with focus on mitochondrial function and energy utilization. WG1
Doerrier Velasco Carolina Doerrier Velasco Carolina Austria Garcia-Roves PM (ES) Inter-laboratory harmonization of protocols to set a final consensus for the evaluation of the technical skills needed for working with permeabilized skeletal muscle. WG1, WG2
Duicu Oana Maria Romania Gnaiger E (AT) MitoFit Coaching Days WG4
Marisol Fernandez-Orriz

Fernandez-Ortiz Marisol

Spain Gnaiger E (AT) Harmonization of respirometric protocols comparing isolated mitochondria and permeabilized muscle fibers WG1, WG2, WG4
Giovarelli Matteo Italy Gnaiger E (AT) Interlaboratory comparison of skeletal muscle fibers High Resolution Respirometry WG2
Hidalgo-Gutierrez Agustin Spain Chakrabarti L (UK) Mitochondrial proteomics in mouse models of CoQ deficiency: therapeutic approaches and aging - completed WG1
Isola Raffaella

Isola Raffaella

Italy Dubouchaud Herve (FR) Mitochondrial oxidative capacity in heart mitochondria after selective AMPK deletion WG2
Jaskiewicz Anna Poland De Palma C (IT) Short-Term Scientific Missions MitoEAGLE WG2
Lelcu Theia Romania Labieniec-Watala M (PL) CA15203 MitoEAGLE WG4
Labieniec-Watala Magdalena

Labieniec-Watala Magdalena

Poland Gnaiger E (AT) Training in the Oroboros MitoFit Laboratory – from SUIT protocols to interpretation and reporting WG1, WG2, WG3, WG4
Ledo Ana Margarida Portugal Juhasz L (HU) Respirometric Protocols for Intact Tissue Preparations - Regulation of mitochondrial respiration by bioactive gases. WG1
Michalak Slawomir

Michalak Slawomir

Poland Gnaiger E (AT) MitoFit Coaching Days WG4
Muntane Jordi

Muntane Jordi

Spain Cervinkova Zuzana (CZ) Regulation of the mitochondria respiration and ROS production during antitumoral Sorafenib-treatment in HepG2 cells WG3
Nozickova Katerina

Nozickova Katerina

Poland Gnaiger E (AT) IOC 124 Schroecken AT WG1, WG4
Manuela Passrugger

Passrugger Manuela

Austria Lalic N (RS) Quality control assessment of mitochondrial respiration- completed WG1
Saric Ana

Saric Ana

Croatia Armand AS (FR) Role of mitochondria in satellite cell activation and differentiation modulated by the calcineurin/NFATc2 pathway: Implications of cellular bioenergetics, respiration and autophagy fluxes WG1, WG2
Saura-Esteller Jose Spain Duchen MR (UK) Study of mitochondrial form and function after fluorizoline treatment WG2
Siewiera Karolina

Siewiera Karolina

Poland Gnaiger E (AT) Fluorecence LED2 Module - training in the Oroboros MitoFit Laboratory WG4
Beata Velika

Velika Beata

Slovakia Gnaiger E (AT) Mitochondrial respiratory function of cryopreserved PBMC - completed WG1, WG4


STSM Grant Period 1

Awardees 2016 November 20th and 2017 March 25th
STSM applicant Country Host Project WG
Dymkowska Dorota Poland Gnaiger E (AT) Training in the MitoFit Laboratory - completed WG1
Timea Komlodi

Komlodi Timea

Hungary Gnaiger E (AT) Standardization of protocols in mitochondria respirometry and fluorometry - completed WG1
Andras Meszaros

Meszaros Andras

Hungary Gnaiger E (AT) Harmonizing protocols of high resolution respirometry and fluorometry - completed WG1
Zanou Nadege Switzerland Garcia-Roves Pablo M (ES) Role of Ryanodin receptor (RyR) 1 in skeletal muscle adaptations to exercise: physiology and metabolism - completed WG1, WG2
  • 4 STSMs for 750.- EUR
3,000.- EUR (Work and Budget Plan)
  • Notification of final decision by the Management Committee: 2016-11-12
  • 2 STSMs for 750.- EUR
1,500.- EUR (addition from FSAC - open for further applications)


STSM Coordinators

Coordinator Magda.JPG Magdalena Labieniec-Watala, PhD, DSc - magdalena.labieniec@biol.uni.lodz.pl


Co-coordinator: Susanne Klaus, Prof. Dr. - klaus@dife.de


COST criteria for evaluation of STSM applications

Aims and scope of a STSM

1. Support individual mobility.
2. Specifically contribute to the scientific objectives of MitoEAGLE.
3. Strengthen the MitoAGLE network.
4. Foster collaborations by allowing researchers participating in the COST Action MitoEAGLE to visit an institution/organisation in
5. Learn new techniques.
6. Gain access to specific data, methods and instruments not available in the home institutions / organisations.


Researchers eligible for a STSM

  • PhD students and Early Career Investigators (up to 8 years after the date of obtaining the PhD/doctorate, full-time equivalent) have priority.
  • Senior scientists.
  • We particularly encourage the application of researchers from “inclusiveness target countries” (Bosnia and Herzegovina, Bulgaria, Croatia, Cyprus, Czech Republic, Estonia, FYR Macedonia, Hungary, Latvia, Lithuania, Luxembourg, Malta, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Turkey).
  • Gender balance will be monitored.
  • The home institution is the institution / organisation where the STSM applicant holds their primary affiliation as registered on their e-COST profile / STSM application / C.V. and where they are currently performing their main strand of research.
  • The host institution concerns the institution / organisation that will host the successful applicant.

Funding regulations

  • STSM in which practical science is getting carried out.
  • STSM which directly lead to tangible outcomes (e.g. writing papers/grants).
  • Funding rule in section 7 of the COST Vademecum.


The money will be returned AFTER the Grantee has completed her/his visit and submitted a report (a written report on the activities carried out during the STSM within 30 days of the end of the research visit)
Researchers from Participating *Inclusiveness Target Country (ITC)can receive 50% of the grant upon completion of the 1st day of the STSM. The remaining 50% will be paid after the Grantee has completed her/his visit and submitted a report.
Note: STSM grantees must make their own arrangements for all provisions related to personal security, health, social security and pension matters.


Funding regulations

Researchers from Participating Inclusiveness Target Country (ITC) can receive 50% of the grant upon completion of the 1st day of the STSM
Early Career Investigators (PhD + 8 years)
allows for:
  • an extended time frame of between 91 days and 180 days
50-70% of the STSM budget will be devote 5to complete specific WG tasks towards a manuscript. Participants will be mainly invited to apply for STSM to join such workshops.
30-50% of the STSM budget will be still available for training activities.

How to apply for a STSM

Interested researchers have to submit their application and supporting documents to the STSM Coordinator:Magdalena Labieniec-Watala

Supporting documents

  1. Letter of invitation to the applicant from a senior researcher affiliated to the host institution.
  2. The submitted STSM application form (downloadable when the online application is submitted – see point 4 below).
  3. Motivation letter including an overview of the proposed activities that will be performed which must contain a plan of work for the visit highlighting the proposed contribution to the scientific objectives of MitoEAGLE.
  4. Letter of support from the home institution.
  5. Full C.V. (including a list of academic publications – if applicable).

Application process

  1. Read the funding rules in Section 7 of the COST Vademecum: »Guidelines
  2. Register for an e-COST profile at https://e-services.cost.eu/ - add your bank account details to your profile.
  3. Include in your application a letter of invitation from the host institution confirming that the host can undertake the STSM on the given dates. The final dates of the STSMs have to be negotiated between the applicant and the host.
  4. Complete, submit and download your STSM application online at: www.cost.eu/STSM.
  5. Send the submitted STSM application form and the supporting documents to the STSM Coordinator:

Evaluation process

  1. The amounts granted for each individual STSM will be determined during the evaluation process by the STSM Coordinator, Chair and Co-chair.
  2. The selection of applicants is based on the scientific scope of the STSM application which must clearly compliment the overall objectives of MitoEAGLE outlined in the Memorandum of Understanding.
  3. Within 30 days from the end date of the STSM, the successful applicant must submit a scientific report to the host institution and to the STSM Coordinator. The Scientific Report must contain the following:
  • purpose of the STSM
  • description of the work carried out during the STSM
  • description of the main results achieved
  • future collaboration with the Host institution (if applicable)
  • foreseen publications/articles resulting from the STSM (if applicable)
  • confirmation by the Host institution of the successful execution of the STSM
  1. Failure to submit the scientific report within 30 days from the end date of the STSM will effectively cancel the Grant.
Please note that the COST Association can request additional information to substantiate the information contained within the documents submittedby STSM applicant.

General: STSM duration and budget

  • Five to 90 days.
  • Early Career Investigators: up to 180 days.
  • STSMs need to be carried out in their entirety within a single Grant Period
  • The financial support is a contribution to the overall expenses and does not necessarily cover all expenses. Financial support is limited to cover travel, accommodation and meal expenses and is paid in the form of a Grant.
  • Up to a maximum of EUR 160 per day for accommodation and meal expenses; up to a maximum of EUR 2,500.- per STSM.
  • for ECIs, a maximum amount of EUR 3500 can be afforded to the Grantee for STSMs with a duration of between 91 and 180 days – For ECIs partaking STSMs with a duration of between 5 and 90 days, the limit of EUR 2 500 must be respected;
  • Financial support is limited to cover travel, accommodation and meal expenses and is paid in the form of a Grant.


MitoEAGLE host institutions

  • We would like to invite institutions who are willing to host MitoEAGLE Short Term Scientific Missions to join the list as an important information for potential applicants. Please, let us know the topics of expertise of your institution related to the MitoEAGLE Working Groups and Memorandum of Understanding, and the contact details of the responsible mentor.
  • Host institutions have to be:
  1. from another COST country, or
  2. an approved near neighbour country (NNC) institution, or
  3. an approved international partner country (IPC) institution, or
  4. an approved EC / EU Agency / an approved European RTD Organisation or an approved International Organisation.
» Open doc file: STSM Host Institution - invitation
» Open doc file: STSM Host Institution - template


Country Host Institution Mentors Working Group Techniques in the lab
AT Medical University Innsbruck Gnaiger E WG1, WG2, WG3, WG4 High-resolution respirometry, O2k-Fluorometry; MitoEAGLE proficiency test, SUIT reference protocols; isolation of mitochondria from various tissues, intact and permeabilized cells, tissue homogenates, permeabilized fibres; mitochondrial physiology data repositories
AT OROBOROS INSTRUMENTS Doerrier Velasco CA, Drinnan M, Gnaiger E, Sumbalova Z WG1, WG2, WG3, WG4 High-resolution respirometry, O2k-Fluorometry; MitoEAGLE proficiency test, SUIT reference protocols; isolation of mitochondria from various tissues, intact and permeabilized cells, tissue homogenates, permeabilized fibres; mitochondrial physiology data repositories
BE University of Louvain (UCL) Medical School Sonveaux P WG1
CZ Charles University Cervinkova Z WG1, WG3 Liver cells, Liver regeneration, Toxic liver injury in vivo and in vitro
DE Technical University of Munich Klingenspor M,Fromme T WG1, WG3, WG4 Preparation of single cell suspensions / isolated mitochondria from cultured cells / murine tissues / human blood, respirometry (Oroboros Oxygraphs, Seahorse Flux Analyzer, legacy clark type electrodes), fluorescence based assays of membrane potential and superoxide production, wide array of molecular biological and biochemical methodology.
DE University Hospital Regensburg Renner-Sattler K, Kreutz M WG1, WG4 Isolation and differentiation of human immune cells,(lymphocyte populations, monocytes, dendritic cells)metabolic analysis of human immune cells and cultured cells respirometry, PreSens technology, Seahorse Technology, measurement of metabolites as glucose and lactate by enzymatic assays, western blot analysis of mitochondrial proteins, determination of mitochondrial content, ROS production and membrane potential by FACS analysis;
DE Institut für Vegetative Physiologie der Universität Köln Wiesner RJ WG1 Mouse genetics, physiology, proteomics, tissue function
DE Heinrich Heine University Ventura N WG2, WG3 We primarily use the nematode c.elegans for aging and toxicology study. We carry out: silencing of nuclear encoded mitochondrial genes, stress response assays, lifespan and neuromuscular assays

further details can be found on our webpage www.iuf-duesseldorf.com/ventura-lab.html

EE Tallinn University of Technology Vendelin M, Birkedal R WG1, WG2 Experiments are mainly performed on isolated mouse cardiomyocytes (WT and KOs). We have been using earlier rat and trout cardiomyocytes as well. As a preparation we use either intact cells or saponin-permiabilized cells. From the techniques routinely used in the lab: oxygraphy (Strathkelvin with custom software); absorbance and fluorescence spectrophotometers; confocal [imaging and FCS/RICS] and fluorescence microscopy. Fluorescence microscopy is running on our custom software allowing us to combine fluorescence imaging with single cell mechanics (control over cell mechanical contraction via carbon fibers) and patch clamping (electrophysiology). [WG2]

In addition to wet lab, we have experience and are actively developing mathematical models of different aspects of heart function: bioenergetics, mechanical contraction, and electrophysiology [WG2]. Finally, all experiments are monitored via database developed in the lab. [WG1]

EE National Institute of Chemical Physics and Biophysics Tepp K, Kaambre T WG1, WG2, WG3 1) Oroboros 2k (4), one with fluorescence module. 2) Mitochondrial respirometry of permeabilized cells, fibers, and isolated mitochondria; Analysis of kinetic parameters of respiration and energy transfer in cell. 3) Analysis of energy transport pathways (creatine and adenylate kinase pathway) 4) Western blotting 5) Immunohistochemistry and imaging ( confocal microscopy).
EG Helmy Institute of Medical Science Ali SS, Abdel-Rahman EA WG2, WG3, WG4 1. Metabolic Studies (two Oroboros O2k with fluorescence and ISE modules + Seahorse XF24 Flux Analyzer), 2. Reactive Oxygen Species detection, identification, and quantifications (Magnettech Benchtop electron paramagnetic resonance spectrometer with temperature and gas controllers + BMG multiplate reader for optical detections + WPI Free Radical Analyzers), Flowcytometric detections in suspended cells, 3. Cell Fractionation techniques (mitochondrial and synaptosomes isolations, isolations of platelets neutrophils from human blood, etc.), 4. Flow cytometry (Attune® Acoustic Focusing Flow Cytometer), 5. Molecular and Cell Biology techniques (Western Blot, RT-PCR, etc.), 6. Imaging (Fluoresence and confocal microscopy), 7. Membrane fluidity measurements by EPR spin labeling.
ES INIBIC A Coruña Mayan MD WG1, WG3, WG4 Cell culture, molecular, cellular and electrophysiological thecniques. Human primary cells (chondrocytes, dermal fibroblast, keratinocytes), cell lines and animal models (mice).
ES INIBIC-Hospital, Universitario A Coruña. Blanco FJ WG1, WG2, WG4 *Metabolic studies (Seahorse XFp8 Extracellular flux analyzer), * ROS production by flow cytometry, * NO production by Greise reactive, * Flow cytometry deteccion with several technicals to detect Apoptotic cells, Δψm, mitochondrial mass…; * ATP production; * Molecular and cell biology technique (PCR, RT-PCR, WB, …); * Imaging by fluorescence microscopy. * cell culture (primary cultures and cell lines)”
ES University of Barcelona Garcia-Roves PM, Perales JC WG1, WG2, WG3, WG4 High-resolution respirometry - different types of culture cells (normal and cancer)

- animal tissues (isolated and homogenized from mice): skeletal muscle, adipose tissue, liver, brain, heart

ES Universitat de Lleida Boada J WG1, WG2, WG3, WG4 Oxygraph 2k, GC-MS, LC-MS-Several SUIT protocols in intact and permeabilized cells and tissues
ES Instituto de Biomedicina de Valencia (IBV-CSIC) Casado Pinna M WG1, WG3
ES Vall D’Hebron Research Institute Villena JA WG3
ES Universitat Autònoma de Barcelona Quintana A WG3 Cell-type specific translatome, trascriptome analyses, animal behavior, mitochondrial dynamics
ES Instituto de Biomedicina de Sevilla Muntane J WG3, WG4
ES Instituto Teofilo Hernando I+D del Medicamento Cano-Abad MF WG3 Neuronal cell culture, mitocondrial calcium measurements by Aequorin and fluorecents probes genetically encoded to the mitocondrial matrix pericam.
ES Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM) Eide L WG3, WG4 We work mainly on the cardiovascular system (endothelium) and in the liver, human blood cells and tissues and on the regulation of mitochondrial function and its impact on disease
ES Universitat of Granada Acuna-Castroviejo D, Escames G, Lopez Garcia LC WG2 Crude and purified mitochondrial preparations; ATP production determination; high resolution respirometry in isolated mitochondria and permeabilized fibers from miocardial and skeletal muscles; in vivo respirometry in zebrafish embryos; spectrophotometric measurement of cytochromes;sSpectrophotometric measurement of mitochondrial respiratory complexes activities; HPLC-EC and HPLC-UV determination of Coenzyme Q and adenosine nucleotides; RT-PCR (including mtDNA copy number), Western-Blot, histology and immunohistochemistry (including COX and SDH stains), oxidative stress markers (carbonyl groups, lipid peroxidation, glutathione redox state, activities of GPx, GRd and MnSOD, ...); mouse models of diseases including aging, sepsis, Parkinson's disease, mitochondrial diseases. Primary cell models of mitochondrial diseases; mouse phenotyping (locomotor activity, MRI and MRS).
ES Severo Ochoa Molecular Biology Center Cadenas S WG2, WG4 Routine respiration, leak, oxphos, uncoupled respiration, non-mitochondrial respiration; Heart biopsies, cultured cells; Diseases models: Ischemia-reperfusion
FR Université Paris-Descartes Petit PX WG1, WG2, WG4 (Oroboros Oxygraphs, Seahorse Flux Analyzer, legacy clark type electrodes)… TPP+ etc… - Image analysis (Opera, Amnis flow image cytometry and flow cytometry (cell shorters and cell analysers).Xcelligence systems for cel proliferation and toxicology, biochemistry and classical signal transduction
FR University Paris-Descartes Armand AS WG1, WG2 Oroboros(Frederic Bouillaud)Seahorse (INEM)- Skeletal muscles, stem cells, nuclear factor NFATC2, cell biology and molecular biology, developmental biology, flow cytometry (analysis and sorting) and confocal
GR National & Kapodistrian University of Athens Trougakos IP WG1, WG2, WG3, WG4
HU Semmelweis University Chinopoulos C WG3, WG4 i) Mitochondrial respirometry in intact and permeabilized cells and isolated mitochondria; ii) fluorescence measurements of mitochondrial membrane potential in intact and permeabilized cells and isolated mitochondria; iii) assessment of mitochondrial substrate-level phosphorylation in intact and permeabilized cells and isolated mitochondria; iv) Western blotting; v) isolation of mitochondria; vi) cell culturing; vii) TR technique protocol for assessment of permeability transition in intact and permeabilized cells and isolated mitochondria; viii) assessment of ANT activity in permeabilized cells and isolated mitochondria; ix) determination of maximum calcium uptake capacity in permeabilized cells and isolated mitochondria.
HU University of Debrecen Bai P WG2, WG3 Cellular models for white adipocytes, beige adipoctes, hepatocytes, skeltal muscle myoblasts/fibers, Oroboros oxygraph 2k for oxymetry on cells, Seahorse XF96 for oxymetry on cells, Flow cytometric determination of mitochondrial membrane potential, Assessment of mitochondrial fusion by microscopy, Gene expression studies, Assessment of the activity of certain cellular energy sensors (AMPK, mTORC1, mTORC2, etc.)
IL Technion - Israel Institute of Technology Ben-Shachar D WG3
IL University of Valencia Borras C, Vina J WG2, WG3, WG4 Oxidative stress-related parameters determination (HPLC, western blotting), Seahorse measurements (just starting with this), molecular biology (RT-PCR, ELISA…), mitochondrial isolation, cell cultures
IR Trinity Biomedical Sciences Institute (TBSI) Porter RK WG1
IR Dublin City University O'Gorman D WG2 Oroboros, Standard lab techniques (western blot, enzyme assay), Proteomics, Gene array, Microscopy (inverted, confocal, etc), Cell culture, Clinical facility (human data collection, including biopsies), Flow cytometry
IT University of Bologna Genova ML WG2, WG4 Tissue fractionation and mitochondrial isolation techniques, First model OROBOROS High resolution Oxygraph and respirometric equipments of different brands, Gel electrophoresis apparatus for BN-PAGE, Spectrophotometric techniques for respiratory enzyme kinetic assays
IT Fondazione S.Lucia -IRCCS Pieroni L WG2, WG3, WG4 Mitochondria enrichment from tissues and cells ( with the possibility to culture cell lines), Wet Lab Biochemistry ( SDS-PAGE, Western Blot, IP, immunohistochemistry)

Proteomics Facilities: MALDI TOF MS/MS-MS: for PMF analysis on digested proteins or Biomarker Profiling in biofluids ( liquor, plasma, urines, etc..), LC-MS and LC-MS/MS: label free comparative proteomics, bottom up and top down analysis, targeted proteomics, GC-MS: metabolic survey analysis

IT University Hospital “Luigi Sacco” De Palma C, Pirkmajer S WG2 Mitochondrial respirometry in intact and permeabilized cells, isolated mitochondria and single muscle fibers; Western blotting; isolation of mitochondria from muscle and BAT; cell culturing (primary muscle stem cells and many different cell lines); real-time PCR (including mtDNA); muscle istology (including SDH and COX stainings and analysis of fiber type composition); measure of in vivo muscle performance; imaging (fluorescence and confoncal microscopy); flow cytometry; mouse models of muscle disease including Duchenne Muscular Dystrophy.
IT University of Verona Calabria E WG4 Separation and isolation of PBMCs, HRR.
IT Università Politecnica delle Marche Battino M WG3, WG4 Mitochondrial Respiration monitored by SeaHorse XF24
LT Lithuanian University of Health Sciences Borutaite V, Arandarcikaite O WG2, WG3, WG4 Isolation of mitochondria from various tissues (brain, heart, liver, kidney), respirometry (isolated mitochondria and cells), measurement of mitochondrial permeability transition, activities of mitochondrial enzymes, cell culture techniques (primary neuronal-glial, cell lines - macrophages, glioblastoma and neuroblastoma)
LV Latvian Institute of Organic Synthesis Makrecka-Kuka M, Liepins E WG1, WG2 mt respirometry combined with fluorometry, enzyme activity measurements, radiolabeled substrate oxidation, molecular biology.

research of mitochondrial function after ischemia-reperfusion injury (ex vivo), in the models of oxidative stress and diabetes.

NL Academic Medical Center, Amsterdam Wuest RC, Houtkooper RH WG1, WG2, WG4 Mitochondrial respiration (Oroboros and Seahorse)Metabolomics, Cell culture, Metabolic disease models (large biobank with fibroblasts from patients), C. elegans, CRISPR/CAS9, Enzyme and DNA diagnostics, Cardiac cell function measurements, stable isotope flux measurements
NL Delft University of Technology McMillan D WG1 Membrane preparation and membrane protein purification, proton-pumping assays (ATP synthase/cytochromes), lipid purification/handling, advanced reconstitution methods, tethered lipid bilayers, Biomimetic membrane technologies, Bioelectrochemistry for membrane-bound quinone activation and membrane analysis (impedance spectroscopy /cyclic volammetry/chronoamperometry), Membrane-protein/soluble protein interactions (QCM-D), quinone-lipid-protein interactions, micro-second time resolution freeze-quench and nano-spectroscopy (rapid pre-steady-state kinetics of proteins), single-molecule ATP synthase rotation, respirometry, flurorescence spectroscopy, mitochondrial isolation
NL Wageningen University Keijer J WG2, WG3, WG4 Molecuar biology etc,Immunohistochemistry etc, Seahorse 96, Oroboros 2K, Indirect calorimetry, Ventilated hood (see: www.molecularphysiology.eu)
NO University of Oslo Rustan AC WG2, WG4 Multiwell fuel handling system using radiolabeled precursors
NO Institute of Microbiology Eide L WG1 mtDNA characterization: mutagenesis and mtDNA integrity assessment.Respiration analyses, mtDNA repair characterization
PL University of Lodz Labieniec-Watala M, Watala C WG1, WG2, WG3, WG4 mt bioenergetics -mt-membrane potential with TPP electrode and fluorescence module (safranin) - Ca2+ level measurements (fluorescence module) - ATP production
PL Poznan University of Medical Sciences Michalak S WG1, WG4 ELISA, Western blotting, indirect fluorescence, enzymology
PT University of Coimbra Palmeira C WG1, WG2, WG3, WG4
RS Faculty of Medicine University of Belgrade Lalic N, Markovic I, |Krako Jakovljevic N WG3, WG4 High-Resolution Respirometry (Oroboros Oxygraph 2k), Western Blot, Flow Cytometry, Transfection via Improved Electroporation (Lonza Nucleofector Technology)
SE Stockholm University Nedergaard J WG1, WG3
SE Lund University, Mitochondrial Medicine Elmer E, Aasander Frostner E WG4 OROBOROS 2k: intact cells protocols, permeabilized cells and tissues protocols, isolated mitochondria and homogenates. Titration-Injection microPump TIP2k and Fluorescence LED2 Module available.Seahorse XFe96: intact cells protocols (OCR, ECAR), Citrate synthase measurements, lactate production etc.
SI University of Ljubljana Mars T, Pirkmajer S WG2, WG4 Human skeletal muscle cell cultures, skeletal muscle cell lines, co-cultures of human skeletal muscle cells and embryonic rat spinal cord (innervated cultured myotubes), human skeletal muscle biopsies, real-time PCR, Western blot, gene silencing (siRNA), ELISA, MAGPIX multiplex (Luminex), flow cytometry, and metabolic assays in cell culture.
SK Slovak Academy of Sciences Ukropec J, Ukropcova B WG2, WG4 primary muscle cell culture, skeletal muscle & adipose tissue biopsy, O2k respirometry (muscle,.blood cells, muscle cell culture - could be established), real-time PCR, western blot (in-cell western), muscle IHCH, exercise intervention studies, clinical metabolic phenotyping, indirect calorimetry, human muscle functional characteristics, aerobic fitness test, physical activity monitoring
UK University of Nottingham Chakrabarti L WG2, WG3 Proteomics, lipidomics with collaborators at UoN, enzyme biochemistry (electron transport chain and others), lifespan studies in c.elegans
UK De Montfort University Moisoi N WG1, WG3, WG4 Models: Cell culture and Drosophila models of Parkinson’s Disease

Techniques: High Resolution Respirometry, RT-qPCR, Western Blotting, Immunofluorescence (Microscopy), Measurements of Neurotransmiters by electrochemical detection (HPLC)

UK University of Cambridge Murray AJ WG1, WG2, WG3, WG4 HRR, enzyme activity assays, isolated heart perfusion, metabolomics, molecular biology
UK University of Cambridge Murray AJ WG1, WG2, WG3, WG4 HRR, enzyme activity assays, isolated heart perfusion, metabolomics, molecular biology
UK University College London Duchen MR WG4 Confocal imaging, fluorescence lifetime imaging and anisotropy measurements; NADH imaging and spectroscopy; EPR and ESR measurements (Prof Chris Kay); super-resolution imaging microscopy; 2photon imaging microscopy; IPScell technology; respirometry (Oroboros and Seahorse); FACS
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