Solinas 2014 Mech Ageing Dev

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Solinas P, Fujioka H, Radivoyevitch T, Tandler B, Hoppel CL (2014) Aging effects on oxidative phosphorylation in rat adrenocortical 3 mitochondria. Mech Ageing Dev 138:10-4.

» PMID:24486556

Solinas P, Fujioka H, Radivoyevitch T, Tandler B, Hoppel CL (2014) Mech Ageing Dev

Abstract: Does aging in itself lead to alteration in adrenocortical mitochondrial oxidative phosphorylation? Mitochondria from Fischer 344 (F344) rats (6 and 24 months old), Brown Norway rats (6 and 32 months old) and F344-Brown Norway hybrid rats (6 and 30 months old) were compared. Mitochondria were isolated from extirpated adrenal cortex. The yields of mitochondria were quantitatively similar in all rat strains irrespective of age. In order to assess the activity of each mitochondrial complex, several different substrates were tested and the rate of oxidative phosphorylation measured. Aging does not affect mitochondrial activity except in the F344 rat adrenal cortex where the maximal ADP-stimulated oxidative phosphorylation decreased with age. We hypothesize that impaired synthesis of steroid hormones by the adrenal cortex with age in F344 rats might be due to decreased adrenocortical mitochondrial oxidative phosphorylation. We conclude that aging results in adrenocortical mitochondria effects that are non-uniform across different rat strains.

Keywords: Mitochondria, Adrenal cortex, Oxidative phosphorylation, Aging, Fischer 344 rat

O2k-Network Lab: US OH Cleveland Hoppel CL


Labels: MiParea: Respiration, mt-Awareness 


Organism: Rat  Tissue;cell: Kidney  Preparation: Isolated mitochondria 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, CIV, NS  HRR: Oxygraph-2k