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• De Araujo 2015 Cold Spring Harb Protoc-A
  ...mberti G, Huber LA (2015) Isolation of early and late endosomes by density gradient centrifugation. Cold Spring Harb Protoc 2015(11):pdb.prot083444. ...iven buffer. Separation will also depend on the medium used to prepare the gradient, whether it is sucrose (the most common) or an alternative. Here we describ
  1 KB (154 words) - 17:19, 13 January 2016
• Thayer 1975 J Biol Chem
  ...f adenosine triphosphate by an artificially imposed electrochemical proton gradient in bovine heart submitochondrial particles. J Biol Chem 250:5330-5. ...p> gradient (15%) alone. These results demonstrate that an electrochemical gradient of protons can drive the synthesis of ATP by reversal of the proton-translo
  2 KB (234 words) - 09:56, 27 November 2015
• Moyes 1988 Am J Physiol
  ...t that carp red muscle mitochondria defend an appropriate mitochondrial pH gradient with temperature-induced changes in intracellular pH.
  2 KB (258 words) - 09:11, 9 November 2016
• Valinomycin
  ...lectrogenic K⁺ transport down the electrochemical transmembrane gradient (150 ng^.mg^-1 protein).
  395 bytes (52 words) - 11:57, 9 June 2023
• Wikstroem 2020 Commun Biol
  ...ATP/ADP.Pi ratio, and mitochondria reach "State 4" with an elevated proton gradient, the degree of coupling drops substantially. The mechanistic cause and the
  2 KB (225 words) - 18:55, 27 January 2024
• Lamberti 2015 Cold Spring Harb Protoc
  ...rophage early and late endosomes by latex bead internalization and density gradient centrifugation. Cold Spring Harb Protoc 2015(12):pdb.prot083451. ...t method for separating endocytic organelles from macrophages on a sucrose gradient, taking advantage of the significantly lower density of the organelles cont
  1 KB (146 words) - 16:10, 9 November 2016
• Lambert 2004 Biochem J
  ...production by NADH:ubiquinone oxidoreductase (complex I) depends on the pH gradient across the mitochondrial inner membrane. Biochem J 382:511-7. ...ng reverse electron transport was at least 3-fold more sensitive to the pH gradient than to the membrane potential.
  2 KB (254 words) - 00:17, 18 February 2022
• Lehr 2021 Methods Mol Biol
  ...commonly used isolation procedure for mitochondria utilizing differential (gradient) centrifugation and depict major challenges to achieve "functional" mitocho |keywords=Differential gradient centrifugation, Isolation of mitochondria, Mitochondrial integrity
  1 KB (193 words) - 17:28, 24 April 2023
• Bailleul 2015 Photosynth Res
  ...neration of an electrochemical proton gradient (Δμ +H ), which comprises a gradient of electric potential (ΔΨ) and of proton concentration (ΔpH). The Δμ +
  1 KB (195 words) - 15:01, 8 August 2023
• Tolleter 2011 Plant Cell
  ...ler M, Peltier G (2011) Control of hydrogen photoproduction by the proton gradient generated by cyclic electron flow in Chlamydomonas reinhardtii. Plant Cell ...s released in the pgrl1 mutant. Regulation of the trans-thylakoidal proton gradient by monitoring pgrl1 expression opens new perspectives toward reprogramming
  2 KB (323 words) - 15:31, 8 August 2023
• Brierley 1994 J Bioenerg Biomembr
  ...+ antiport keeps matrix [Na+] low and the Na(+)-gradient equal to the H(+)-gradient. A Na+/Ca2+ antiport provides a Ca(2+)-extruding mechanism that permits the
  1 KB (197 words) - 10:42, 16 February 2022
• Fisar 2016a Folia Biol (Praha)
  ...y the isolation procedure was the greatest after purification in a sucrose gradient. We confirmed that pig brain mitochondria can serve as a biological model f
  2 KB (321 words) - 17:57, 9 November 2017
• Wikstroem 2023 FEBS Lett
  ...l gradient across the inner mitochondrial or bacterial cell membrane. This gradient is the primary source of energy for aerobic synthesis of ATP. Oxidation of
  2 KB (236 words) - 18:50, 27 January 2024
• Scalar
  ...differences of concentration or pressure are scalars, whereas a potential gradient is a [[vector]]. Similarly, the [[protonmotive force]] and metabolic oxygen
  523 bytes (64 words) - 05:27, 16 September 2022
• Vector
  ...' is a pysicochemical quantity with magnitude and spatial direction of a [[gradient]]. Symbols for vectors are written in bold face. For example, [[velocity]],
  521 bytes (64 words) - 05:24, 16 September 2022
• Ortiz-Prado 2019 Am J Blood Res
  ...s supply within the human body, including a facilitated description of the gradient of pressure from the atmosphere to the cells. This review also included the
  2 KB (240 words) - 09:24, 28 June 2022
• Free activity
  2 KB (275 words) - 10:14, 13 October 2022
• Klusch 2017 Elife
  ...nine between the two channels prevents proton leakage. The steep potential gradient over the sub-nm inter-channel distance exerts a force on the deprotonated g
  2 KB (220 words) - 17:22, 16 January 2021
• Morre 1989 Biotechniques
  ...ducible and facile. It is especially useful for tissue culture cells since gradient methods often are complicated by alterations in plasma membrane density wit
  2 KB (252 words) - 22:35, 16 February 2021
• Bryant 2018 Thesis
  ...hrough uncoupling proteins (UCPs) which can cause a decrease in the proton gradient independent of the production of ATP. Here, I investigate changes in UCPs a
  3 KB (411 words) - 16:37, 28 March 2018

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