Ukropec 2017 MITOEAGLE Obergurgl

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COST Action MitoEAGLE
Could functional state of muscle mitochondria reflect exercise-induced changes in insulin sensitivity, glucose tolerance and cognitive performance in individuals with mild cognitive impairment?

Link: MitoEAGLE

Ukropec J, Nemec M, Maderova D, Tirpakova V, Krumpolec P, Slobodova L, Schoen M, Sumbalova Z, Cvecka J, Vician M, Sedliak M, Ukropcova B (2017)

Event: MitoEAGLE Obergurgl 2017

COST Action MIitoEAGLE

Sedentary aging accelerates the risk of chronic metabolic and neurodegenerative diseases, while regular exercise could effectively support healthy ageing. Here we report initial results of the 3-month supervised aerobic/strength training intervention in seniors with/without mild cognitive and/or metabolic impairment.

Whole body glucose metabolism was examined with oGTT, insulin sensitivity by euglycemic hyperinsulinemic clamp, Resting Energy Expenditure (REE) and metabolic substrate preference (RQ) by indirect calorimetry (Ergostik, Geratherm). Cognitive functions were assessed with a battery of validated cognitive tests (MMSE, CogState, Memtrax). Biopsy of m. vastus lateralis was performed in local anesthesia using Bergström needle technique. Functional state of muscle mitochondria was measured by Oroboros Oxygraph-2k high-resolution respirometry applying SUIT protocols RP1 and RP2. Oxygen consumption (pmol.s-1.mg-1 tissue wet weight) was evaluated in saponin-permeabilized muscle fibers.

Mitochondrial respiration was significantly reduced in a subgroup of individuals with impaired glucose tolerance. Moreover, muscle mitochondrial oxidative phosphorylation (OXPHOS) capacity was negatively associated with BMI (R=–0.548; p=0.042) and mitochondrial fatty acid oxidation was positively associated with short-term memory test score (CogState, R=0.616; p=0.019). Insulin-induced change in resting energy expenditure (∆REE, clamp) was positively associated with both coupled (R=0.797; p=0.018) and noncoupled (R=0.717; p=0.045) muscle mitochondrial respiration rate. Results of the 3-months exercise intervention on whole body and muscle specific metabolism and mitochondrial function will be presented during the meeting (we have finalized the data collection at this moment).

Our preliminary results showed that functional state of muscle mitochondria is tightly linked with BMI, whole body metabolic state and cognitive performance in seniors with mild cognitive impairment. Effects of regular exercise induced-changes in whole body metabolic and muscle mitochondrial function on cognitive performance in seniors will be presented.


Bioblast editor: Kandolf G O2k-Network Lab: AT Innsbruck Oroboros, SK Bratislava Sumbalova Z


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Aging;senescence, Diabetes, Neurodegenerative 


Tissue;cell: Skeletal muscle  Preparation: Permeabilized cells 


Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, Gp, NS  HRR: Oxygraph-2k  Event: A1, Oral 


Affiliations

(1)Ukropec J, (1)Nemec M, (1)Maderová D, (2)Tirpakova V, (1)Krumpolec P, (3)Slobodová L, (3)Schön M, (4,5)Sumbalová Z, (6)Cvecka J, (7)Vician M, (6)Sedliak M, (1,2,6)Ukropcova B
  1. Inst Experimental Endocrinol, Biomedical Research Center Slovak Acad Sciences
  2. Inst Physical Education, Slovak Health Univ
  3. Inst Pathol Physiol, Fac Medicine Comenius Univ
  4. Pharmacobiochem Lab, Fac Medicine Comenius Univ; Bratislava, Slovakia
  5. Daniel Swarovski Research Lab, Dept Visceral, Transplant Thoracic Surgery, Medical Univ Innsbruck, Austria
  6. Fac Physical Education Sports, Comenius Univ
  7. Dept Surgery, Slovak Medical Univ; Bratislava, Slovakia.- jozef.ukropec@gmail.com

Support

Grant support: VEGA 2/0191/15; APVV-15-0253; SAS-NSC JRC 2013/17