Varela-Lopez 2017 MiPschool Obergurgl

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COST Action MitoEAGLE
Mitochondrial function impairment after Duxorubicin treatment is attenuated by strawberry intake in old and young rats.

Link: MitoEAGLE

Varela-Lopez A, Forbes-Hernandez TY, Gasparrini M, Reboredo-Rodriguez P, Afrin S, Cianciosi D, Giampieri F (2017)

Event: MiPschool Obergurgl 2017

COST Action MitoEAGLE

Doxorubicin action is closely linked production of free radicals and the increase of oxidative stress, which implies both anti-tumor and toxic effects. Fruit and vegetables consumption is associated with a lower incidence of several chronic pathologies, many of them associated with elevate oxidative stress. In particular, strawberry contains many dietary components including vitamins, minerals, and many phytochemical compounds that could be responsible of such effects [1-3]. The aim of the present study was to evaluate the in vivo effects of strawberries consumption on mitochondrial functionality and oxidative stress levels on young/old rats exposed to oxidative conditions as consequence of doxurubicin treatment

For this porpouse, 32 young and 32 old male rats were randomly divided into two groups and maintained on standard (25 g) AIN93 diet, or modified diets where 15% of the total calories were substituted with ice-dried strawberry fruit for two months. After that, half of individuals where submitted to 2 intra-peritoneal injections of doxorubicin used as inducer of oxidative stress 24 hours prior to sacrifice. Then, two parameters were studied, total ROS concentration that measured with EPR spectrometry and oxygen consumption rate (OCR) that was measured by seahorse XF24 to assess liver mitochondrial functionality.

In comparison with the control group, strawberry fed rats showed a marked improvement of OCR and a relevant decrease of ROS levels. Doxorubicin-treated rats fed the control diet showed a decrease in OCR and a increase in ROS levels. However, strawberry comsumption led to a restoration of OCR and a decrease of ROS concentration to values similar to those found inuntreated animals receiving the control diet.

Overall, these results confers to strawberry a potential health benefit against oxidative stress, particularly under high oxidative stress conditions including aging. Notwithstanding, it is needed to clarify where ROS levels contribute to mitochondrial dysfunction or ROS levels depend on mitochondrial function. In this context addtional data about the status of other antioxidant defense (endogenous and exogenous) systems will also result interesting.


Bioblast editor: Kandolf G


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Pharmacology;toxicology  Pathology: Aging;senescence 

Organism: Rat  Tissue;cell: Liver 




Event: D2, Poster 


Affiliations

Varela-Lopez A(1), Forbes-Hernandez TY(2), Gasparrini M(3), Reboredo-Rodriguez P(4), Afrin S(3), Cianciosi D(3), Giampieri F(3)
  1. Dept Physiol, Univ Granada, Spain
  2. Univ Intern Iberoamericana (UNINI), Campeche, Mexico
  3. Dept Scienze Cliniche, Univ Politecnica delle Marche, Ancona, Italy
  4. Dept Analyical Food Chemistry, Univ Vigo, Ourense, Spain.- alvarela@ugr.es

Figures

Varela-Lopez Figure1 MiPschool Obergurgl 2017.jpg

Figure 1. Effects of strawberry on oxygen consumption in liver mitochondria of young (a) and old (b) rats.






Varela-Lopez Figure2 MiPschool Obergurgl 2017.jpg

Figure 2. Rate of ROS generation measured by the kinetics of CM• accumulation in liver mitochondria of young (a) and old (b) rats at 37 °C.


References

  1. Giampieri F, Alvarez-Suarez JM, Battino M (2014) Strawberry and human health: effects beyond antioxidant activity. J Agric Food Chem 62:3867–76.
  2. Alvarez-Suarez JM, Giampieri F, Tulipani S, Casoli T, Di Stefano G, González-Paramás AM, Santos-Buelga C, Busco F, Quiles JL, Cordero MD, Bompadre S, Mezzetti B, Battino M (2014) One-month strawberry-rich anthocyanin supplementation ameliorates cardiovascular risk, oxidative stress markers and platelet activation in humans. J Nutr Biochem 25:289-94.
  3. Amatori S, Mazzoni L, Alvarez-Suarez JM, Giampieri F, Gasparrini M, Forbes-Hernandez TY, Afrin S, Provenzano AE, Persico E, Mezzetti B, Amici A, Mirco Fanelli, Battino M (2013) Polyphenol-rich strawberry extract (PRSE) shows in vitro and in vivo biological activity against invasive breast cancer cells. J Berry Res 3:169–79.


Support

Recepient of a MitoEAGLE scholarship.