SUIT-001 O2 mt D001: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=FNSGp(PGM) | |abbr=FNSGp(PGM) | ||
|description=[[File:1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-. | |description=[[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp-.png|400px|SUIT-RP1]] | ||
|info='''A: SUIT_RP1''' - [[MiPNet21.06 SUIT RP]] | |info='''A: SUIT_RP1''' - [[MiPNet21.06 SUIT RP]] | ||
}} | }} | ||
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== Harmonized SUIT protocols == | == Harmonized SUIT protocols == | ||
[[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama; | '''SUIT RP1''' [[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd.png|400px|SUIT-RP1]] | ||
[[File:1D; | '''SUIT RP2''' [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd.png|500px|SUIT-RP2]] | ||
:::: '''SUIT RP1''':1PM;2D:2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10Tm''';11Azd | :::: '''SUIT RP1''':1PM;2D:2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10Tm''';11Azd | ||
:::: [[1D; | :::: [[1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot-|SUIT RP2]]:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azd | ||
[[File: | [[File:RP1&RP2.png|400px]] '''Harmonization between RP1 and RP2''' | ||
RP1 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Pathway control state|pathway control states]]. | RP1 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see [[Digitonin]]). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet [[Pathway control state|pathway control states]]. | ||
:::: '''SUIT RP1 in cells''':'''ce1''';1Dig;1PM;2D:2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10Tm''';11Azd | :::: '''SUIT RP1 in cells''':'''ce1''';1Dig;1PM;2D:2c;3U;4G;5S;6Oct;7Rot;'''8Gp''';9Ama;'''10Tm''';11Azd | ||
:::: [[1D;2OctM;3P;4G;5S;6Gp;7U;8Rot-#Harmonized_SUIT_protocols|SUIT RP2 in cells]]:'''ce1''';1Dig;1D; | :::: [[1D;2OctM;3P;4G;5S;6Gp;7U;8Rot-#Harmonized_SUIT_protocols|SUIT RP2 in cells]]:'''ce1''';1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;'''10Rot''';11Ama;'''12AsTm''';13Azdd | ||
[[File:RP1&RP2_pce(Dig).png|400px]] '''Harmonization between RP1 and RP2 in cells''' | |||
== SUIT O2 DLP for RP1 (DatLab 7.1) == | == SUIT O2 DLP for RP1 (DatLab 7.1) == |
Revision as of 16:32, 27 November 2017
Description
Abbreviation: FNSGp(PGM)
Reference: A: SUIT_RP1 - MiPNet21.06 SUIT RP
MitoPedia concepts:
SUIT protocol,
SUIT A
- MitoPedia: SUIT - SUIT reference protocol RP1
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: orthogonal
Harmonized SUIT protocols
- SUIT RP1:1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2
RP1 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet pathway control states.
- SUIT RP1 in cells:ce1;1Dig;1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2 in cells:ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd
Harmonization between RP1 and RP2 in cells
SUIT O2 DLP for RP1 (DatLab 7.1)
- DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols MitoPedia: SUIT. Instrumental DL-protocols are displayed in the DL-Protocol library.
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