Carboxyatractyloside: Difference between revisions
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:::: '''Comment:''' CAT is considered to be much more specific than [[atractyloside|Atractyloside]] (Atr), and the effective concentration required is much much lower (ยตM). With Atr, a very high concentration (mM) is required for full inhibition. | :::: '''Comment:''' CAT is considered to be much more specific than [[atractyloside|Atractyloside]] (Atr), and the effective concentration required is much much lower (ยตM). With Atr, a very high concentration (mM) is required for full inhibition. CAT appears to be little permeable to intact cells and can thus only be applied in permeabilized cells and tissues or in isolated mitochondria ([[Ruas_2016_PLOS_ONE|Ruas 2016 PLOS_ONE]]). | ||
ย ยป [[Talk:Carboxyatractyloside]] | ย ยป [[Talk:Carboxyatractyloside]] |
Latest revision as of 14:55, 7 February 2024
Description
Carboxyatractyloside CAT is a highly selective and potent inhibitor of the adenine nucleotide translocator (ANT). CAT stabilizes the nucleoside binding site of ANT on the cytoplasmic (positive) side of the inner membrane and blocks the exchange of matrix ATP and cytoplasmic ADP. It causes stabilization of the c conformation of ANT leading to permeability transition pore (PTP) opening, loss of mitochondrial membrane potential, and apoptosis.
Abbreviation: CAT
Reference: MiPNet03.02
Application in HRR
- CAT: Carboxyatractyloside (carboxyatractyloside potassium salt from Xanthium sibiricum; C31H46O18S2*xK+), Sigma-Aldrich: C4992, store at -20 ยฐC, CAS: 77228-71-8, M = 802.99 gยทmol-1
- Hazard statements: H301, H311, H331; toxic if swallowed, toxic in contact with skin, toxic if inhaled
- CAT: Carboxyatractyloside (carboxyatractyloside potassium salt from Xanthium sibiricum; C31H46O18S2*xK+), Sigma-Aldrich: C4992, store at -20 ยฐC, CAS: 77228-71-8, M = 802.99 gยทmol-1
- Alternative source: Merck 216201-5MG (Calbiochem, Carboxyatractyloside potassium salt from Xanthium sibiricum, C31H43O18S2K3*3H2O), 5 mg, store at -20 ยฐC, M = 939.1 gยทmol-1.
- Preparation of 2 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- For a vial with 2 mg of carboxyatractyloside: dissolve in 1065 ยตL H2O.
- Divide into 100 ยตL aliquots.
- Store at -20 ยฐC.
- ยป O2k manual titrations ยป MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1-5 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 1-5 ยตM.
- Preparation of 0.5 mM solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 50 ยตL of the 2 mM stock solution.
- Add 150 ยตL H2O.
- ยป O2k manual titrations ยป MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1-5 ยตL using a 10 ยตL Hamilton syringe.
- Final concentration: 1-5 ยตM.
- Comment: CAT is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (ยตM). With Atr, a very high concentration (mM) is required for full inhibition. CAT appears to be little permeable to intact cells and can thus only be applied in permeabilized cells and tissues or in isolated mitochondria (Ruas 2016 PLOS_ONE).
ยป Talk:Carboxyatractyloside
MitoPedia topics:
Inhibitor