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| '''Preparation of 500 mM ADP stock solution''' (dissolved in H<sub>2</sub>O):
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| ::1) weigh 501.3 mg of ADP (FW = 501.3) = (1 mmol ADP)
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| ::2) add 1.2 ml H<sub>2</sub>OΒ (ADP is not dissolved at this stage.
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| ::3) neutralize with 5 N KOH (approx. 450 Β΅l)! ADP will dissolve after addition of KOH!
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| ::4) check pH and adjust to 7 if necessary
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| ::5) adjust final volume to 2 ml and divide into 0.2 ml portions
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| ::6) store frozen at -80 Β°C
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| '''Preparation of 500 mM ADP stock solution with 3 mM free Mg<sup>2+</sup>''' (dissolved in H<sub>2</sub>O):
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| ToΒ keep free [Mg<sup>2+</sup>] constant during respirationΒ measurement mix ADP with MgCl<sub>2</sub> (0.6 molΒ MgCl<sub>2</sub>/mol ADP).
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| '''MgCl<sub>2</sub>''' (Scharlau MA0036: MgCl<sub>2</sub>.6H<sub>2</sub>O, FW = 203.3)
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| ::1) weigh 501.3 mg of ADP (FW = 501.3) = (1 mmol ADP)
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| ::2) add 1.2 ml H<sub>2</sub>OΒ (ADP is not dissolved at this stage.
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| ::3) neutralize with 5 N KOH (approx. 450 Β΅l)! ADP will dissolve after addition of KOH!
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| ::4) add 121,98 mg MgCl<sub>2</sub> .WhiteΒ precipitate will occur, which will be dissolved after 1-2 min ofΒ stirring on magnetic stirrer at room temperature!
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| ::5) check pH and adjust to 7 if necessary
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| ::6) adjust final volume to 2 ml and divide into 0.2 ml portions
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| ::7) store frozen at -80 Β°C
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| == ADP accessed == | | == ADP accessed == |
| [[ADP]] (D) has been accessed more than | | [[ADP]] (D) has been accessed more than |
| *Β 5,000 times (2012-10-12) | | *Β 5,000 times (2012-10-12) |