Talk:ADP

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Former Instructions

These preparation instructions are still correct. Ordering ADP in portions of 1 g per vial turned out to be very useful and is therefore the recommended strategy (as described on the ADP main page).

Preparation without MgCl₂ and lower amounts of ADP

Preparation of 500 mM ADP stock solution (dissolved in H₂O):

1. Weigh 501.3 mg of ADP = 1 mmol ADP
2. Add 1.2 mL H₂O (ADP is not dissolved at this stage).
3. Neutralize with 5 M KOH (approx. 450 µL). ADP will dissolve after addition of KOH.
4. Check pH and adjust to 7 with 5 M KOH if necessary.
5. Adjust final volume to 2 mL and divide into 0.2 mL portions.
6. Store at -80 °C.

Preparation with MgCl₂ and lower amounts of ADP

Preparation of 500 mM ADP stock solution with 300 mM free Mg²⁺ (dissolved in H₂O):

1. Weigh 501.3 mg of ADP = 1 mmol ADP.
2. Add 1.2 mL H₂O (ADP is not dissolved at this stage).
3. Neutralize with 5 M KOH (approx. 450 µl). ADP will dissolve after addition of KOH.
4. Add 121.98 mg MgCl₂. White precipitate will occur, which will dissolve during 1-2 min stirring on magnetic stirrer at RT.
5. Check pH and adjust to 7 with 5 M KOH if necessary.
6. Adjust final volume to 2 mL and divide into 0.2 mL portions.
7. Store at -80 °C.