MitoKit-CII/Succinate-nv: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Snv | |abbr=Snv | ||
|description='''NV118/MitoKit-CII''' (diacetoxymethyl succinate) is a plasma membrane-permeable | |description='''NV118/MitoKit-CII''' (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing succinate in the intracellular space. | ||
|info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]] | |info=[[MitoKit-CII]], [[MitoKit-CII MitoPedia]], [[Ehinger 2016 Nat Commun]] | ||
}} | }} | ||
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:::: '''Snv: NV118''' 25 mg, MW=262.2 g.mol<sup>-1</sup> | :::: '''Snv: NV118''' 25 mg, MW=262.2 g.mol<sup>-1</sup> | ||
:::: '''Preparation of 1 M stock solution:''' | :::: '''Preparation of 1 M stock solution:''' | ||
::::# Dissolve 25 mg NV118 in 95. | ::::# Dissolve 25 mg NV118 in 95.3 ยตL DMSO. | ||
:::: '''Preparation of 100 mM stock solution (from the 1M stock):''' ย | :::: '''Preparation of 100 mM stock solution (from the 1M stock):''' ย | ||
::::# Dilute 95. | ::::# Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO. | ||
::::# Divide into 50 ยตL portions.ย ย | ::::# Divide into 50 ยตL portions.ย ย | ||
::::# Store at -20 ยฐC. | ::::# Store at -20 ยฐC. |
Revision as of 11:12, 18 April 2019
Description
NV118/MitoKit-CII (diacetoxymethyl succinate) is a plasma membrane-permeable prodrug that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing succinate in the intracellular space.
Abbreviation: Snv
Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
Application in HRR
- Snv: NV118 25 mg, MW=262.2 g.mol-1
- Preparation of 1 M stock solution:
- Dissolve 25 mg NV118 in 95.3 ยตL DMSO.
- Preparation of 100 mM stock solution (from the 1M stock):
- Dilute 95.3 ยตL 1 M stock solution with 857.7 ยตL DMSO.
- Divide into 50 ยตL portions.
- Store at -20 ยฐC.
- ยป O2k manual titrations
- Titration volume: 1 ยตL steps using a 10 ยตL syringe (2 mL O2k-Chamber).
- Final concentration: 50 ยตM steps.
- Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
- ยป O2k manual titrations