Talk:Brewer GJ: Difference between revisions
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It is challenging to isolate from cultured cells enough mitochondria for high-resolution respirometry.ย Then you will always wonder how the mitochondria were affected by the isolation and whether you obtained a representative fraction.ย Instead we developed techniques for high-resolution respirometry with the OROBOROS O2k of neuronal mitochondria in situ, with mitochondria in their normal environment inside neurons attached to a substrate.ย Here are our results: | It is challenging to isolate from cultured cells enough mitochondria for high-resolution respirometry.ย Then you will always wonder how the mitochondria were affected by the isolation and whether you obtained a representative fraction.ย Instead we developed techniques for high-resolution respirometry with the OROBOROS O2k of neuronal mitochondria in situ, with mitochondria in their normal environment inside neurons attached to a substrate.ย Here are our results: | ||
Jones TT, Brewer GJ (2009) Critical age-related loss of cofactors of neuron cytochrome c oxidase reversed by estrogen.ย Exp Neurology 215: 212-9. [[Jones_2009_ExpNeurol|ยปPM:18930048 PMCID:3018880]] | |||
Jones TT, Brewer GJ (2010) Age-related deficiencies in Complex I endogenous substrate availability and reserve capacity of Complex IV in cortical neuron electron transport.ย Biochim Biophys Acta Bioenergetics 1797: 167-76. [[Jones_2010_Biochim Biophys Acta|ยปPM:19799853ย PMCID: 2812684]] | |||
Latest revision as of 00:10, 30 October 2014
High-resolution respirometry with attached neuronal cells
From: Mitochondria Interest Group [1] From Gregory J. Brewer
Sent: 26 September 2011 18:53
Re: [MITOCHONDRIA-L] functional mitochondria isolation from primary cortical cell cultures
Content of this Email is solely the Responsibility of the Author.--------
Dr. Castello and MIG,
It is challenging to isolate from cultured cells enough mitochondria for high-resolution respirometry. Then you will always wonder how the mitochondria were affected by the isolation and whether you obtained a representative fraction. Instead we developed techniques for high-resolution respirometry with the OROBOROS O2k of neuronal mitochondria in situ, with mitochondria in their normal environment inside neurons attached to a substrate. Here are our results:
Jones TT, Brewer GJ (2009) Critical age-related loss of cofactors of neuron cytochrome c oxidase reversed by estrogen. Exp Neurology 215: 212-9. ยปPM:18930048 PMCID:3018880
Jones TT, Brewer GJ (2010) Age-related deficiencies in Complex I endogenous substrate availability and reserve capacity of Complex IV in cortical neuron electron transport. Biochim Biophys Acta Bioenergetics 1797: 167-76. ยปPM:19799853 PMCID: 2812684
Please let me know if you need further assistance acquiring this information.
Grace to you,
Gregory J. Brewer, Ph.D., Professor
Kenneth Stark Endowed Chair in Alzheimer Research
Departments of Neurology and
Medical Microbiology, Immunology and Cell Biology
Southern Illinois University Sch. Med.
P.O. Box 19626
Springfield, IL 62794-9626
825 N. Rutledge Street, Room 2640
Springfield, IL 62702
http://www.siumed.edu/mmi/faculty/brewer.html
217-545-5230
FAX 217-545-3227