Description
The ET-pathway coupling efficiency (E-L coupling control factor) is a normalized flux ratio, jβE = βE/E = (E-L)/E = 1-L/E. jβE is 0.0 at zero coupling (L=E) and 1.0 at the limit of a fully coupled system (L=0). The background state is the LEAK state which is stimulated to ET-pathway reference state by uncoupler titration. LEAK states LN or LT may be stimulated first by saturating ADP (State P) with subsequent uncoupler titration to State E. The ET-pathway coupling efficiency is based on measurement of a coupling control ratio (LEAK control ratio, L/E), whereas the thermodynamic or ergodynamic efficiency of coupling between ATP production (DT phosphorylation) and oxygen consumption is based on measurement of the output/input flux ratio (~P/O2 ratio) and output/input force ratio (Gibbs force of phosphorylation/Gibbs force of oxidation). Biochemical coupling efficiency is either expressed as the ET-pathway coupling efficiency, jβE, or OXPHOS coupling efficiency, jβP, obtained in a coupling control protocol (phosphorylation control protocol). Β» MiPNet article
Abbreviation: jβE
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
Biochemical coupling efficiency: from 0 to <1
Gnaiger E (2015) Biochemical coupling efficiency: from 0 to <1. Mitochondr Physiol Network 2015-01-18. |
Abstract: Zooming in on biochemical coupling efficiency, jβE compared to jβP.
β’ O2k-Network Lab: AT Innsbruck Gnaiger E
Labels:
Regulation: Coupling efficiency;uncoupling
Coupling state: LEAK, ET-pathway"ET-pathway" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Theory
- Quantification of coupling of mitochondrial respiration is a fundamental component of OXPHOS analysis.[1],[2] Biochemical coupling efficiency is distinguished from ergodynamic efficiency.[3],[4],[5]
Coupling control states for jβE
- Β» Respiratory state, ETS-competent pathway control state, Electron transfer system
- Reference state, ZX: ET capacity, E = EΒ΄-ROX
- Background state, YX: LEAK respiration, L = LΒ΄-ROX
- Metabolic control variable, X=ZX-YX: Free ET-capacity, βE = E-L
- Β» Respiratory state, ETS-competent pathway control state, Electron transfer system
Flux control ratio and flux control factor
- Β» Flux control ratio, FCR, Flux control factor, FCF
- Coupling control ratio, YX/ZX: LEAK control ratio (L/E coupling control ratio), L/E
- Coupling control factor, 1-YX/ZX: ETS coupling efficiency: jβE = βE/E =(E-L)/E = 1-L/E
- Β» Flux control ratio, FCR, Flux control factor, FCF
Compare
mt-Preparations
- OXPHOS coupling efficiency, P-L control factor: jβP = βP/P = (P-L)/P = 1-L/P
- netOXPHOS control ratio, βP/E control ratio: βP/E = (P-L)/E
- OXPHOS capacity, P = PΒ΄-ROX
Intact cells
- ROUTINE coupling efficiency, (R-L or βR control factor): jβR = βR/R = (R-L)/R = 1-L/R
- netROUTINE control ratio, βR/E control ratio: βR/E = (R-L)/E
- ROUTINE respiration, R = RΒ΄-ROX
References
- β Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. Β»Open AccessΒ«
- β Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. Β»UncouplerΒ«
- β Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. Β»Open AccessΒ«
- β Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109. Β»Bioblast AccessΒ«
- β Gnaiger E (2015) Cell ergometry: OXPHOS and ET-pathway coupling efficiency. Mitochondr Physiol Network 2015-01-18. Β»Bioblast linkΒ«
Coupling control factors: biochemical efficiencies
- OXPHOS coupling efficiency, (P-L or βP control factor): jβP = βP/P = (P-L)/P = 1-L/P
- ROUTINE coupling efficiency: jβR = βR/R =(R-L)/R = 1-L/R
- ETS coupling efficiency, E-L coupling control factor: jβE = βE/E = (E-L)/E = 1-L/E
Coupling control factors: apparent excess capacity factors
- Excess E-P capacity factor, E-P coupling control factor: jExP = (E-P)/E = 1-P/E
- Excess E-R capacity factor, E-R coupling control factor: jExR = (E-R)/E = 1-R/E
Coupling control ratios
- Β» Coupling control ratio
- L/P coupling control ratio: L/P
- L/R coupling control ratio, L/R
- LEAK control ratio, L/E
- OXPHOS control ratio, P/E
- ROUTINE control ratio, R/E
- netOXPHOS control ratio, βP/E control ratio: βP/E = (P-L)/E
- netROUTINE control ratio, βR/E control ratio: βR/E = (R-L)/E