From Bioblast
Description
NV118/MitoKit-CII (diacetoxymethyl succinate) is a plasma membrane-permeable (p) prodrug that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing succinate in the intracellular space.
Abbreviation: Snv
Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
Application in HRR
- Snv: NV118 25 mg, MW=262.2 g.mol-1
- Preparation of 1 M stock solution:
- Dissolve 25 mg NV118 in 95.1 Β΅L DMSO.
- Preparation of 100 mM stock solution (from the 1M stock):
- Dilute 95.1 Β΅L 1 M stock solution with 855.9 Β΅L DMSO.
- Divide into 50 Β΅L portions.
- Store at -20 Β°C.
- Β» O2k manual titrations
- Titration volume: 1 Β΅L steps using a 10 Β΅L syringe (2 mL O2k-Chamber).
- Final concentration: 50 Β΅M steps
- Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
- Β» O2k manual titrations