- high-resolution terminology - matching measurements at high-resolution
Additive effect of convergent electron flow
Description
There is an additive effect on respiration by electron flow converging at the Q-junction from respiratory Complexes I and II (CI+II e-input), glycerophosphate dehydrogenase and electron-transferring flavoprotein. Convergent electron flow corresponds to the operation of the TCA cycle and mitochondrial substrate supply in vivo. Convergent electron flow simultaneously through CI+II into the Q-junction supports higher OXPHOS capacity and ETS capacity than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI+II e-input are required for reconstitution of intracellular TCA cycle function. The convergent CI+II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with mt-preparations have underestimated cellular OXPHOS capacities, due to the gating effect through a single branch, corresponding to additivity.
Abbreviation: A
Reference: MiPNet12.12, Gnaiger_2009_IJBCB
MitoPedia methods:
Respirometry
Labels:
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Enzyme: Complex I, Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Flux Control; Additivity; Threshold; Excess Capacity"Flux Control; Additivity; Threshold; Excess Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Theory
