Azide: Difference between revisions
From Bioblast
No edit summary |
(replaced FW by M and added units g*mol-1) |
||
| Line 9: | Line 9: | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; | :::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 g·mol<sup>-1</sup>. | ||
::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | ::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | ||
Revision as of 13:19, 12 February 2021
- high-resolution terminology - matching measurements at high-resolution
Azide
Description
Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).
Abbreviation: Azd
Reference: MiPNet03.02
Application in HRR
- Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; M = 65.01 g·mol-1.
- Caution: Very toxic!
- Preparation of 4 M storage and stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 260 mg of sodium azide.
- Dissolve in 1 mL H20.
- Divide into 0.5 mL portions.
- Store at -20 °C.
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): ≥50 µL using a 100 µL Hamilton syringe.
- Final concentration: ≥100 mM.
- Preparation of 2 M stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 400 µL of the 4 M stock solution.
- Add 400 µL H2O.
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): ≥25 µL using a 100 µL Hamilton syringe.
- Final concentration: ≥100 mM.
MitoPedia topics:
Inhibitor
