Brennan 2006 Biochim Biophys Acta: Difference between revisions
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{{Publication | {{Publication | ||
|title=Brennan CM, Breen EP, Porter RK (2006) Cold acclimation and oxygen consumption in the thymus. Biochim | |title=Brennan CM, Breen EP, Porter RK (2006) Cold acclimation and oxygen consumption in the thymus. Biochim Biophys Acta 1757:1463-8. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/17022934 PMID: 17022934] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/17022934 PMID: 17022934 Open Access] | ||
|authors=Brennan CM, Breen EP, Porter RK | |authors=Brennan CM, Breen EP, Porter RK | ||
|year=2006 | |year=2006 | ||
|journal=Biochim | |journal=Biochim Biophys Acta | ||
|abstract=Mitochondrial uncoupling protein 1 is usually associated with brown adipose tissue but has recently been discovered in rat and mouse thymus. We wished to establish whether there was a thermogenic role for UCP 1 in thymus and thus examined the effect of 5 weeks cold-acclimation on rat thymus tissue abundance, thymocyte oxygen consumption, thymus mitochondrial abundance, uncoupling protein 1 expression and function. We found that thymocytes from cold-acclimated rats had oxygen consumption rates 8 times less than those from rats held at room temperature and that thymocytes from cold-acclimated rats or rats kept at room temperature were noradrenaline insensitive. In addition, we found that thymus tissue or mitochondrial abundance was not increased after cold-acclimation. However uncoupling protein 1 expression per unit mass of mitochondria was increased after cold-acclimation, as determined by immunoblotting (not, vert, similar 1.7-fold) and GDP binding (not, vert, similar 1.5-fold). Consistent with our protein expression data, we also observed an increased, state 4 (not, vert, similar 1.5-fold), GDP-inhibitable (not, vert, similar 1.3-fold) and palmitate activatable (not, vert, similar 1.6-fold) oxygen consumption rates in isolated thymus mitochondria. However, extrapolation of our data showed that cold-acclimation only increased the amount of UCP 1 per gram of thymus tissue not, vert, similar 1.2-fold. Taken together, we conclude that UCP 1 does not have a thermogenic role in thymus. | |abstract=Mitochondrial uncoupling protein 1 is usually associated with brown adipose tissue but has recently been discovered in rat and mouse thymus. We wished to establish whether there was a thermogenic role for UCP 1 in thymus and thus examined the effect of 5 weeks cold-acclimation on rat thymus tissue abundance, thymocyte oxygen consumption, thymus mitochondrial abundance, uncoupling protein 1 expression and function. We found that thymocytes from cold-acclimated rats had oxygen consumption rates 8 times less than those from rats held at room temperature and that thymocytes from cold-acclimated rats or rats kept at room temperature were noradrenaline insensitive. In addition, we found that thymus tissue or mitochondrial abundance was not increased after cold-acclimation. However uncoupling protein 1 expression per unit mass of mitochondria was increased after cold-acclimation, as determined by immunoblotting (not, vert, similar 1.7-fold) and GDP binding (not, vert, similar 1.5-fold). Consistent with our protein expression data, we also observed an increased, state 4 (not, vert, similar 1.5-fold), GDP-inhibitable (not, vert, similar 1.3-fold) and palmitate activatable (not, vert, similar 1.6-fold) oxygen consumption rates in isolated thymus mitochondria. However, extrapolation of our data showed that cold-acclimation only increased the amount of UCP 1 per gram of thymus tissue not, vert, similar 1.2-fold. Taken together, we conclude that UCP 1 does not have a thermogenic role in thymus. | ||
|keywords=Uncoupling protein-1,Thymus, Thymocyte, Mitochondria, Purine nucleotide, Oxygen consumption | |keywords=Uncoupling protein-1,Thymus, Thymocyte, Mitochondria, Purine nucleotide, Oxygen consumption | ||
|mipnetlab=IE Dublin Porter RK | |mipnetlab=IE Dublin Porter RK | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Mouse, Rat | |||
|tissues=Islet cell;pancreas;thymus, Fat | |||
|preparations=Intact cells | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Latest revision as of 14:57, 24 March 2015
Brennan CM, Breen EP, Porter RK (2006) Cold acclimation and oxygen consumption in the thymus. Biochim Biophys Acta 1757:1463-8. |
Brennan CM, Breen EP, Porter RK (2006) Biochim Biophys Acta
Abstract: Mitochondrial uncoupling protein 1 is usually associated with brown adipose tissue but has recently been discovered in rat and mouse thymus. We wished to establish whether there was a thermogenic role for UCP 1 in thymus and thus examined the effect of 5 weeks cold-acclimation on rat thymus tissue abundance, thymocyte oxygen consumption, thymus mitochondrial abundance, uncoupling protein 1 expression and function. We found that thymocytes from cold-acclimated rats had oxygen consumption rates 8 times less than those from rats held at room temperature and that thymocytes from cold-acclimated rats or rats kept at room temperature were noradrenaline insensitive. In addition, we found that thymus tissue or mitochondrial abundance was not increased after cold-acclimation. However uncoupling protein 1 expression per unit mass of mitochondria was increased after cold-acclimation, as determined by immunoblotting (not, vert, similar 1.7-fold) and GDP binding (not, vert, similar 1.5-fold). Consistent with our protein expression data, we also observed an increased, state 4 (not, vert, similar 1.5-fold), GDP-inhibitable (not, vert, similar 1.3-fold) and palmitate activatable (not, vert, similar 1.6-fold) oxygen consumption rates in isolated thymus mitochondria. However, extrapolation of our data showed that cold-acclimation only increased the amount of UCP 1 per gram of thymus tissue not, vert, similar 1.2-fold. Taken together, we conclude that UCP 1 does not have a thermogenic role in thymus. โข Keywords: Uncoupling protein-1, Thymus, Thymocyte, Mitochondria, Purine nucleotide, Oxygen consumption
โข O2k-Network Lab: IE Dublin Porter RK
Labels:
Organism: Mouse, Rat
Tissue;cell: Islet cell;pancreas;thymus, Fat
Preparation: Intact cells
HRR: Oxygraph-2k