- high-resolution terminology - matching measurements at high-resolution
Carbonyl cyanide m-chlorophenyl hydrazone
Description
Carbonyl cyanide m-chloro phenyl hydrazone (C9H5ClN4; FW = 204.62) is a protonophore (H+ ionophore) and is used as a chemical uncoupler of oxidative phosphorylation.
Abbreviation: CCCP
Reference: Lou 2007 Biochem J, Skulachev 1998 Biochim Biophys Acta
MitoPedia methods:
Respirometry
MitoPedia topics:
Uncoupler
Application in HRR
MiPNet News CCCP 2013-07-11
- Using CCCP as an uncoupler in our on-going experiments with homogenate preparations of mouse brain and heart, we obtained results on maximum ETS capacity and mt-membrane potential which were identical to parallel experiments using FCCP. Therefore, we replace in our Mitochondrial Research Laboratory FCCP by CCCP. Importantly, CCCP is significantly less expensive than the most frequently used uncoupler FCCP. The optimum CCCP concentration for maximum oxygen flux is only slightly higher than the optimum FCCP concentration. - Eigentler A, Gnaiger E
Preparation
- C: CCCP (Carbonyl cyanide 3-chlorophenylhydrazone, C9H5ClN4O); Sigma C 2759, 100 mg, store at -20 °C: FW = 204.62
- Preparation of 1 mM stock solution (dissolved in DMSO):
- 1) Weigh 1.02 mg of CCCP into a glass vial.
- 2) Dissolve in 5 ml DMSO.
- 3) Divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution.
- 4) Store in glass vials at -20 °C.
- 5) Titrations: 10 µl syringe, 1 µl steps, corresponding to 0.5 µM steps of the concentration in the 2 ml O2k-chamber.
Caution: Toxic!
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
