Cizmarova B

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MiPsociety
COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


 

Cizmarova B


MitoPedia topics: EAGLE 

COST: Member COST WG1: WG1


COST WG4: WG4

COST ECI: ECI

MC Member - Management Committee MitoEAGLE


Name Čižmárová (Velika) Beata, PhD, RNDr.
Institution
Beata Cizmarova

Pavol Jozef Šafárik University in Kosice

Faculty of Medicine
Department of Medical and Clinical Biochemistry

Visiting scientist

  • Research on: Cryopreservation and respiration of blood cells

Beata Cizmarova joined Oroboros in January 2017 within the framework
of the Postdoc Scholarship Action Austria and COST Action MitoEAGLE.

Address Trieda SNP 1, 040 11
City Košice
State/Province
Country Slovakia
Email beata.cizmarova@upjs.sk
Weblink
O2k-Network Lab


Labels:



Publications

 PublishedReference
Gnaiger 2019 MitoFit Preprint Arch2019Gnaiger E, Aasander Frostner E, Abdul Karim N, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v4.
Sumbalova 2018 Recent Advances Mitochondr Med Coenzyme Q102018Sumbalová Z, Garcia-Souza LF, Cizmarova B, Volani C, Gnaiger E (2018) Analysis of mitochondrial function in human blood cells. In: Recent advances in mitochondrial medicine and Coenzyme Q10. Gvozdjáková A, Cornelissen G, Singh RB eds, Nova Sciences:255-68.
Gatterer 2018 J Sports Sci Med2018Gatterer H, Menz V, Salazar-Martinez E, Sumbalova Z, Garcia-Souza LF, Cizmarova B, Gnaiger E, Burtscher M (2018) Exercise performance, muscle oxygen extraction and blood cell mitochondrial respiration after repeated-sprint and sprint interval training in hypoxia: a pilot study. J Sports Sci Med 17:339-347.

Abstracts

 PublishedReference
Evinova 2018 MiP20182018
MiPsociety
High-resolution respirometry of neuroblastoma SH-SY 5Y cell line.
Cizmarova 2018 MiP20182018
Velika Beata
High-resolution respirometry of neuroblastoma SH-SY 5Y cell line.
Garcia-Souza 2018 Life Sciences Meeting 2018 Innsbruck AT2018A respirometric cell viability test for peripheral-blood mononuclear cells and platelets.
Cizmarova 2017 MITOEAGLE Obergurgl2017
Beata Cizmarova
High-resolution respirometry and viability of cryopreserved blood cells.
Cizmarova 2017 Abstract MITOEAGLE Barcelona2017
COST Action MitoEAGLE
Optimization of cryopreservation of human peripheral blood mononuclear cells and platelets for high-resolution respirometry.
Cizmarova 2017 MiP20172017
Beate Cizmarova
PBMC cryopreservation and evaluation for functional mitochondrial diagnosis.
Laner 2017 Abstract EUROMIT2017 Cologne2017
EUROMIT2017
K-Regio MitoFit and MitoEAGLE – towards a global data bank on mitochondrial physiology.
Garcia-Souza 2017 MiP20172017
Luiz Garcia
Short-term acclimatization to moderate altitude does not affect respiration of peripheral blood mononuclear cells and platelets.
Garcia-Souza 2017 MiPschool Obergurgl2017
Luiz Garcia-Souza
Assessment of mitochondrial respiratory function in cryopreserved platelets.
Sumbalova 2017 MiP2017 WG42017
Zuzana Sumbalova
Purity of blood cell fractions in respirometric studies: peripheral blood mononuclear cells and platelets.
Cizmarova 2017 MiPschool Obergurgl2017
Beata Cizmarova
The scope of cryopreservation in blood cells for functional mitochondrial diagnosis.
Garcia-Souza 2017 MITOEAGLE Obergurgl2017
GarciaL.JPG
Towards a database on PBMC mitochondrial respiration: first steps on a literature survey linked to the MitoFit experimental data.


MitoEAGLE Short-Term Scientific Mission

Work Plan summary
My name is Beata Velika and I work as Research specialist at the Pavol Jozef Safarik University in Kosice, Department of Medical and Clinical Biochemistry, Faculty of Medicine, Slovakia. During my study and work I specialized in fluorescence spectroscopy, and I have experience with different isolation methods, including mitochondria isolation, and measuring of their native fluorescence and respiration.
I would like to carry out the project in cooperation with the research group at the Mitochondrial Research Laboratory of Prof. Dr. Erich Gnaiger in Innsbruck, Austria (Medical University of Innsbruck; K-Regio project MitoFit) in collaboration with the scientific company OROBOROS INSTRUMENTS Corp. The laboratory of Prof. Dr. Erich Gnaiger contains very high standard laboratory equipment, with several specialists in mitochondrial physiology field. The short-term project is focused on the optimization of SUIT (substrate-uncoupler-inhibitor titration) protocols for high-resolution respirometry of intact cryopreserved human peripheral blood mononuclear cells (PBMC). The usage of human PBMC will provide a significant stimulus for the entire field of mitochondrial medicine, because the mitochondrial function of PBMC is linked with numerous diseases and metabolic conditions in the human body, and the protocols developed during the project will lead to the standardization of the procedures, and should be used for studying mitochondria physiological and pathological mechanisms. Cryopreserved cells (stored at -80 °C), will be thawed in 37 °C water bath, dilluted in 10 ml pre-warmed 37 °C medium composed of DPBS + 2%FBS and centrifuged (300 g, 6 minutes). The sediment will be resuspended and counted. The suspension of cryopreserved cells will be titrated into the 2-ml chambers of the Oxygraph-2k (Oroboros Instruments, Innsbruck, Austria). We will optimize the SUIT protocols with defined substrate combinations, uncoupler and inhibitor titrations, which will be applied for the measurement of respiration of the cryopreserved cells. The results will be compared with the respiration of the fresh cells (reference). Obtained results will be presented in 10th MiPschool 2017 MitoEAGLE and MitoEAGLE Workshop WG1-4, Obergurgl AT (2017 Jul 23-30).

MitoEAGLE Inclusiveness Target Countries - Conference Grant

Scientific report - MiP2017
Scientific report - WISe3



Participated at


Visiting scientist in the Oroboros MitoFit Laboratory

O2k-Network

Beata Cizmarova : Visiting scientist at the Oroboros MitoFit Laboratory

  • January 08 to 19 2018
  • January 15 to July 31 2017