Description
The ETS coupling efficiency (E-L coupling control factor) is a normalized flux ratio, j≈E = ≈E/E = (E-L)/E = 1-L/E. j≈E is 0.0 at zero coupling (L=E) and 1.0 at the limit of a fully coupled system (L=0). The background state is the LEAK state which is stimulated to ETS reference state by uncoupler titration. LEAK states LN or LT may be stimulated first by saturating ADP (State P) with subsequent uncoupler titration to State E. The ETS coupling efficiency is based on measurement of a coupling control ratio (LEAK control ratio, L/E), whereas the thermodynamic or ergodynamic efficiency of coupling between ATP production (DT phosphorylation) and oxygen consumption is based on measurement of the output/input flux ratio (~P/O2 ratio) and output/input force ratio (Gibbs force of phosphorylation/Gibbs force of oxidation). Biochemical coupling efficiency is either expressed as the ETS coupling efficiency, j≈E, or OXPHOS coupling efficiency, j≈P, obtained in a coupling control protocol (phosphorylation control protocol). » MiPNet article
Abbreviation: j≈E
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
Biochemical coupling efficiency: from 0 to <1
Gnaiger E (2015) Biochemical coupling efficiency: from 0 to <1. Mitochondr Physiol Network 2015-01-18. |
Abstract: Zooming in on biochemical coupling efficiency, j≈E compared to j≈P.
• O2k-Network Lab: AT Innsbruck Gnaiger E
Labels:
Regulation: Coupling efficiency;uncoupling
Coupling state: LEAK, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Theory
- Quantification of coupling of mitochondrial respiration is a fundamental component of OXPHOS analysis.[1],[2] Biochemical coupling efficiency is distinguished from ergodynamic efficiency.[3],[4],[5]
Coupling control states for j≈E
- » Respiratory state, ETS-competent pathway control state, Electron transfer system
- Reference state, ZX: ETS capacity, E = E´-ROX
- Background state, YX: LEAK respiration, L = L´-ROX
- Metabolic control variable, X=ZX-YX: Free ETS capacity, ≈E = E-L
- » Respiratory state, ETS-competent pathway control state, Electron transfer system
Flux control ratio and flux control factor
- » Flux control ratio, FCR, Flux control factor, FCF
- Coupling control ratio, YX/ZX: LEAK control ratio (L/E coupling control ratio), L/E
- Coupling control factor, 1-YX/ZX: ETS coupling efficiency: j≈E = ≈E/E =(E-L)/E = 1-L/E
- » Flux control ratio, FCR, Flux control factor, FCF
Compare
mt-Preparations
- OXPHOS coupling efficiency, P-L control factor: j≈P = ≈P/P = (P-L)/P = 1-L/P
- netOXPHOS control ratio, ≈P/E control ratio: ≈P/E = (P-L)/E
- OXPHOS capacity, P = P´-ROX
Intact cells
- ROUTINE coupling efficiency, (R-L or ≈R control factor): j≈R = ≈R/R = (R-L)/R = 1-L/R
- netROUTINE control ratio, ≈R/E control ratio: ≈R/E = (R-L)/E
- ROUTINE respiration, R = R´-ROX
References
- ↑ Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »Open Access«
- ↑ Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »Uncoupler«
- ↑ Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. »Open Access«
- ↑ Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109. »Bioblast Access«
- ↑ Gnaiger E (2015) Cell ergometry: OXPHOS and ETS coupling efficiency. Mitochondr Physiol Network 2015-01-18. »Bioblast link«
Coupling control factors: biochemical efficiencies
- OXPHOS coupling efficiency, (P-L or ≈P control factor): j≈P = ≈P/P = (P-L)/P = 1-L/P
- ROUTINE coupling efficiency: j≈R = ≈R/R =(R-L)/R = 1-L/R
- ETS coupling efficiency, E-L coupling control factor: j≈E = ≈E/E = (E-L)/E = 1-L/E
Coupling control factors: apparent excess capacity factors
- Excess E-P capacity factor, E-P coupling control factor: jExP = (E-P)/E = 1-P/E
- Excess E-R capacity factor, E-R coupling control factor: jExR = (E-R)/E = 1-R/E
Coupling control ratios
- » Coupling control ratio
- L/P coupling control ratio: L/P
- L/R coupling control ratio, L/R
- LEAK control ratio, L/E
- OXPHOS control ratio, P/E
- ROUTINE control ratio, R/E
- netOXPHOS control ratio, ≈P/E control ratio: ≈P/E = (P-L)/E
- netROUTINE control ratio, ≈R/E control ratio: ≈R/E = (R-L)/E