Difference between revisions of "Gnaiger 1995 J Bioenerg Biomembr"
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|year=1995 | |year=1995 | ||
|journal=J Bioenerg Biomembr | |journal=J Bioenerg Biomembr | ||
|abstract=Control and regulation of mitochondrial and cellular respiration by oxygen is discussed with three aims: (1) A review of intracellular oxygen levels and gradients, particularly in heart, emphasizes the dominance of extracellular oxygen gradients. Intracellular oxygen pressure, | |abstract=Control and regulation of mitochondrial and cellular respiration by oxygen is discussed with three aims: (1) A review of intracellular oxygen levels and gradients, particularly in heart, emphasizes the dominance of extracellular oxygen gradients. Intracellular oxygen pressure, ''p''<sub>O2</sub>, is low, typically one to two orders of magnitude below incubation conditions used routinely for the study of respiratory control in isolated mitochondria. The ''p''<sub>O2</sub> range of respiratory control by oxygen overlaps with cellular oxygen profiles, indicating the significance of ''p''<sub>O2</sub> in actual metabolic regulation. (2) A methodologically detailed discussion of high-resolution respirometry is necessary for the controversial topic of respiratory control by oxygen, since the risk of methodological artefact is closely connected with far-reaching theoretical implications. Instrumental and analytical errors may mask effects of energetic state and partially explain the divergent views on the regulatory role of intracellular ''p''<sub>O2</sub> . Oxygen pressure for half-maximum respiration, ''p''<sub>50</sub>, in isolated mitochondria in the [[LEAK state]] was 0.025 kPa (0.2 Torr; 0.3 µM O<sub>2</sub>), whereas ''p''<sub>50</sub> in endothelial cells was 0.06–0.08 kPa (0.5 Torr). (3) A model derived from the thermodynamics of irreversible processes was developed which quantitatively accounts for near-hyperbolic flux/''p''<sub>O2</sub> relations in isolated mitochondria. The apparent ''p''<sub>50</sub> is a function of redox potential and protonmotive force. The protonmotive force collapses after uncoupling and consequently causes a decrease in ''p''<sub>50</sub>. Whereas it is becoming accepted that flux control is shared by several enzymes, insufficient attention is paid to the notion of complementary kinetic and thermodynamic flux control mechanisms. | ||
|keywords=Oxygen limitation, p<sub>50</sub>, Critical oxygen pressure, Respirometry, Polarographic oxygen sensor, Human endothelial cells, Rat liver mitochondria, Intracellular pO<sub>2</sub>, Oxygen gradients, Kinetics, Nonequilibrium thermodynamics | |keywords=Oxygen limitation, ''p''<sub>50</sub>, Critical oxygen pressure, Respirometry, Polarographic oxygen sensor, Human endothelial cells, Rat liver mitochondria, Intracellular pO<sub>2</sub>, Oxygen gradients, Kinetics, Nonequilibrium thermodynamics | ||
|mipnetlab=AT_Innsbruck_Gnaiger E | |mipnetlab=AT_Innsbruck_Gnaiger E | ||
|articletype=Protocol; Manual | |articletype=Protocol; Manual | ||
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}} | }} | ||
== High-resolution respirometry == | == High-resolution respirometry == | ||
* This publication was the first to establish [[high-resolution respirometry]], standardized oxygen calibration (air and internal zero oxygen calibration), automatic instrumental background correction of oxygen flux, deconvolution of the oxygen signal for time resolution required in oxygen kinetics and quality control of the limit of detection of oxygen flux at | * This publication was the first to establish [[high-resolution respirometry]], based on the [[Oxygraph-2k |OROBOROS Oxygraph]] [1] and integrating with [[DatLab]] [2,3] the standardized oxygen calibration (air and internal zero oxygen calibration), automatic instrumental background correction of oxygen flux, deconvolution of the oxygen signal for time resolution required in oxygen kinetics and quality control of the limit of detection of oxygen flux at ±1 pmol O<sub>2</sub>·s<sup>-1</sup>·ml<sup>-1</sup>. | ||
# Haller T, Ortner M, Gnaiger E (1994) A respirometer for investigating oxidative cell metabolism. Toward optimization of respiratory studies. Analyt Biochem 218:338-42. »[[Haller 1994 Analyt Biochem |PMID: 8074290]] | |||
# Méndez G, Gnaiger E (1994) How does oxygen pressure control oxygen flux in isolated mitochondria? A methodological approach by high-resolution respirometry and digital data analysis. In: What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermo-Kinetics 3. Innsbruck Univ Press:191-4. »[[Mendez 1994 BTK-191 |Open Access]] | |||
# Steinlechner-Maran R, Eberl T, Margreiter R, Gnaiger E (1994) Oxygen dependence of cellular respiration in endothelial cells: A sensitive toxicological test. In: What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermo-Kinetics 3. Innsbruck Univ Press:283-7. »[[SteinlechnerMaran 1994 BTK-283 |Open Access]] | |||
== Follow-up reviews on high-resolution respirometry == | == Follow-up reviews on high-resolution respirometry == | ||
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== Follow-up reviews on mitochondrial and cellular oxygen kinetics == | == Follow-up reviews on mitochondrial and cellular oxygen kinetics == | ||
* [[Gnaiger 1998 Biochim Biophys Acta]], [[Gnaiger 1998 J Exp Biol]] | * [[Gnaiger 1998 Biochim Biophys Acta]], [[Gnaiger 1998 J Exp Biol]] | ||
* [[Gnaiger 2000 Proc Natl Acad Sci U S A]] | |||
* [[Gnaiger 2001 Respir Physiol]] | * [[Gnaiger 2001 Respir Physiol]] | ||
* [[Gnaiger 2002 Biochem Soc Trans]] | * [[Gnaiger 2002 Biochem Soc Trans]] | ||
* [[Gnaiger 2003 Adv Exp Med Biol]] | * [[Gnaiger 2003 Adv Exp Med Biol]] | ||
* [[Scandurra 2010 Adv Exp Med Biol]] | * [[Scandurra 2010 Adv Exp Med Biol]] | ||
Revision as of 13:15, 27 December 2014
| Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. |
Gnaiger E, Steinlechner-Maran R, Mendez G, Eberl T, Margreiter R (1995) J Bioenerg Biomembr
Abstract: Control and regulation of mitochondrial and cellular respiration by oxygen is discussed with three aims: (1) A review of intracellular oxygen levels and gradients, particularly in heart, emphasizes the dominance of extracellular oxygen gradients. Intracellular oxygen pressure, pO2, is low, typically one to two orders of magnitude below incubation conditions used routinely for the study of respiratory control in isolated mitochondria. The pO2 range of respiratory control by oxygen overlaps with cellular oxygen profiles, indicating the significance of pO2 in actual metabolic regulation. (2) A methodologically detailed discussion of high-resolution respirometry is necessary for the controversial topic of respiratory control by oxygen, since the risk of methodological artefact is closely connected with far-reaching theoretical implications. Instrumental and analytical errors may mask effects of energetic state and partially explain the divergent views on the regulatory role of intracellular pO2 . Oxygen pressure for half-maximum respiration, p50, in isolated mitochondria in the LEAK state was 0.025 kPa (0.2 Torr; 0.3 µM O2), whereas p50 in endothelial cells was 0.06–0.08 kPa (0.5 Torr). (3) A model derived from the thermodynamics of irreversible processes was developed which quantitatively accounts for near-hyperbolic flux/pO2 relations in isolated mitochondria. The apparent p50 is a function of redox potential and protonmotive force. The protonmotive force collapses after uncoupling and consequently causes a decrease in p50. Whereas it is becoming accepted that flux control is shared by several enzymes, insufficient attention is paid to the notion of complementary kinetic and thermodynamic flux control mechanisms. • Keywords: Oxygen limitation, p50, Critical oxygen pressure, Respirometry, Polarographic oxygen sensor, Human endothelial cells, Rat liver mitochondria, Intracellular pO2, Oxygen gradients, Kinetics, Nonequilibrium thermodynamics
• O2k-Network Lab: AT_Innsbruck_Gnaiger E
Labels: MiParea: Respiration, Instruments;methods
Stress:Hypoxia Organism: Human, Rat Tissue;cell: Liver, Endothelial; Epithelial; Mesothelial Cell"Endothelial; Epithelial; Mesothelial Cell" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. Preparation: Intact cells, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: ADP, Flux control, O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: ROUTINE, OXPHOS
HRR: Oxygraph-2k
Made history
High-resolution respirometry
- This publication was the first to establish high-resolution respirometry, based on the OROBOROS Oxygraph [1] and integrating with DatLab [2,3] the standardized oxygen calibration (air and internal zero oxygen calibration), automatic instrumental background correction of oxygen flux, deconvolution of the oxygen signal for time resolution required in oxygen kinetics and quality control of the limit of detection of oxygen flux at ±1 pmol O2·s-1·ml-1.
- Haller T, Ortner M, Gnaiger E (1994) A respirometer for investigating oxidative cell metabolism. Toward optimization of respiratory studies. Analyt Biochem 218:338-42. »PMID: 8074290
- Méndez G, Gnaiger E (1994) How does oxygen pressure control oxygen flux in isolated mitochondria? A methodological approach by high-resolution respirometry and digital data analysis. In: What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermo-Kinetics 3. Innsbruck Univ Press:191-4. »Open Access
- Steinlechner-Maran R, Eberl T, Margreiter R, Gnaiger E (1994) Oxygen dependence of cellular respiration in endothelial cells: A sensitive toxicological test. In: What is Controlling Life? (Gnaiger E, Gellerich FN, Wyss M, eds) Modern Trends in BioThermo-Kinetics 3. Innsbruck Univ Press:283-7. »Open Access
