Gregg 2019 J Biol Chem: Difference between revisions
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{{Publication | {{Publication | ||
|title=Gregg T, Sdao SM, Dhillon RS, Rensvold JW, Lewandowski SL, Pagliarini DJ, Denu JM, Merrins MJ (2019) Obesity-dependent CDK1 signaling stimulates mitochondrial respiration at | |title=Gregg T, Sdao SM, Dhillon RS, Rensvold JW, Lewandowski SL, Pagliarini DJ, Denu JM, Merrins MJ (2019) Obesity-dependent CDK1 signaling stimulates mitochondrial respiration at Complex I in pancreatic ฮฒ-cells. J Biol Chem 294:4656-66. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/30700550 PMID: 30700550 Open Access] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/30700550 PMID: 30700550 Open Access] | ||
|authors=Gregg T, Sdao SM, Dhillon RS, Rensvold JW, Lewandowski SL, Pagliarini DJ, Denu JM, Merrins MJ | |authors=Gregg T, Sdao SM, Dhillon RS, Rensvold JW, Lewandowski SL, Pagliarini DJ, Denu JM, Merrins MJ | ||
|year=2019 | |year=2019 | ||
|journal=J Biol Chem | |journal=J Biol Chem | ||
|abstract=ฮฒ-cell mitochondria play a central role in coupling glucose metabolism with insulin secretion. Here, we identified a metabolic function of cyclin-dependent kinase 1 (CDK1)/cyclin B1 - the activation of mitochondrial respiratory | |abstract=ฮฒ-cell mitochondria play a central role in coupling glucose metabolism with insulin secretion. Here, we identified a metabolic function of cyclin-dependent kinase 1 (CDK1)/cyclin B1 - the activation of mitochondrial respiratory Complex I - that is active in quiescent adult ฮฒ-cells and hyperactive in ฮฒ-cells from obese (''ob/ob'') mice. In wild-type islets, respirometry revealed that 65 % of Complex I flux and 49 % of state 3 respiration is sensitive to CDK1 inhibition. Islets from ''ob/ob'' mice expressed more cyclin B1 and exhibited a higher sensitivity to CDK1 blockade, which reduced Complex I flux by 76 % and state 3 respiration by 79 %. The ensuing reduction in mitochondrial NADH utilization, measured with 2-photon NAD(P)H fluorescence lifetime imaging (FLIM), was matched in the cytosol by a lag in citrate cycling, as shown with a FRET reporter targeted to ฮฒ-cells. Moreover, time-resolved measurements revealed that in ''ob/ob'' islets, where Complex I flux dominates respiration, CDK1 inhibition is sufficient to restrict the duty cycle of ATP/ADP and calcium oscillations, the parameter that dynamically encodes ฮฒ-cell glucose sensing. Direct Complex I inhibition with rotenone mimicked the restrictive effects of CDK1 inhibition on mitochondrial respiration, NADH turnover, ATP/ADP, and calcium influx. These findings identify Complex I as a critical mediator of obesity-associated metabolic remodeling in ฮฒ-cells, and implicate CDK1 as a regulator of Complex I that enhances ฮฒ-cell glucose sensing. | ||
</small>Published under license by The American Society for Biochemistry and Molecular Biology, Inc.</small> | </small>Published under license by The American Society for Biochemistry and Molecular Biology, Inc.</small> | ||
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|tissues=Islet cell;pancreas;thymus | |tissues=Islet cell;pancreas;thymus | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|enzymes=Complex I | |||
|couplingstates=LEAK, OXPHOS, ET | |couplingstates=LEAK, OXPHOS, ET | ||
|pathways=N, S, NS, ROX | |pathways=N, S, NS, ROX |
Latest revision as of 09:25, 7 November 2019
Gregg T, Sdao SM, Dhillon RS, Rensvold JW, Lewandowski SL, Pagliarini DJ, Denu JM, Merrins MJ (2019) Obesity-dependent CDK1 signaling stimulates mitochondrial respiration at Complex I in pancreatic ฮฒ-cells. J Biol Chem 294:4656-66. |
Gregg T, Sdao SM, Dhillon RS, Rensvold JW, Lewandowski SL, Pagliarini DJ, Denu JM, Merrins MJ (2019) J Biol Chem
Abstract: ฮฒ-cell mitochondria play a central role in coupling glucose metabolism with insulin secretion. Here, we identified a metabolic function of cyclin-dependent kinase 1 (CDK1)/cyclin B1 - the activation of mitochondrial respiratory Complex I - that is active in quiescent adult ฮฒ-cells and hyperactive in ฮฒ-cells from obese (ob/ob) mice. In wild-type islets, respirometry revealed that 65 % of Complex I flux and 49 % of state 3 respiration is sensitive to CDK1 inhibition. Islets from ob/ob mice expressed more cyclin B1 and exhibited a higher sensitivity to CDK1 blockade, which reduced Complex I flux by 76 % and state 3 respiration by 79 %. The ensuing reduction in mitochondrial NADH utilization, measured with 2-photon NAD(P)H fluorescence lifetime imaging (FLIM), was matched in the cytosol by a lag in citrate cycling, as shown with a FRET reporter targeted to ฮฒ-cells. Moreover, time-resolved measurements revealed that in ob/ob islets, where Complex I flux dominates respiration, CDK1 inhibition is sufficient to restrict the duty cycle of ATP/ADP and calcium oscillations, the parameter that dynamically encodes ฮฒ-cell glucose sensing. Direct Complex I inhibition with rotenone mimicked the restrictive effects of CDK1 inhibition on mitochondrial respiration, NADH turnover, ATP/ADP, and calcium influx. These findings identify Complex I as a critical mediator of obesity-associated metabolic remodeling in ฮฒ-cells, and implicate CDK1 as a regulator of Complex I that enhances ฮฒ-cell glucose sensing.
Published under license by The American Society for Biochemistry and Molecular Biology, Inc. โข Keywords: Complex I, RO-3306, Calcium, Cyclin B1, Cyclin-dependent kinase 1 (CDK1), Insulin secretion, Mitochondrial metabolism, ob/ob mice, Obesity, Pancreatic beta cell โข Bioblast editor: Plangger M โข O2k-Network Lab: US WI Madison Denu JM
Labels: MiParea: Respiration
Pathology: Diabetes, Obesity
Organism: Mouse Tissue;cell: Islet cell;pancreas;thymus Preparation: Permeabilized tissue Enzyme: Complex I
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k
2019-02