Hervouet 2006 Biochem Biophys Res Commun: Difference between revisions
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{{Publication | {{Publication | ||
|title=Hervouet E, Pecina P, Demont J, Vojtíšková A, Simonnet H, Houštek J, Godinot C (2006) Inhibition of cytochrome c oxidase subunit 4 precursor processing by the hypoxia mimic cobalt chloride. Biochem Biophys Res Commun | |title=Hervouet E, Pecina P, Demont J, Vojtíšková A, Simonnet H, Houštek J, Godinot C (2006) Inhibition of cytochrome ''c'' oxidase subunit 4 precursor processing by the hypoxia mimic cobalt chloride. Biochem Biophys Res Commun 344:1086-93. | ||
|authors=Hervouet E, Pecina P, Demont J, | |authors=Hervouet E, Pecina P, Demont J, Vojtiskova A, Simonnet H, Houstek J, Godinot C | ||
|year=2006 | |year=2006 | ||
|journal=Biochem Biophys Res Commun | |journal=Biochem Biophys Res Commun | ||
|abstract=Cobalt is often used as a hypoxia mimic in cell culture, because it stabilizes the α subunits of the transcription factor, HIF (hypoxia-inducible factor). We have previously shown that HIF stabilization due to a deficiency of the von Hippel Lindau protein (pVHL) in clear cell renal carcinoma (CRCC) was correlated to a down-regulation of oxidative phosphorylation. To better understand this mechanism, we have used CoCl2 in CRCC expressing stably transfected vhl. We show that, in addition to its effect on HIF-α subunits, CoCl2 prevented the normal processing of the precursor of cytochrome c oxidase (COX) subunit 4 and induced COX degradation very likely by inhibiting the mitochondrial intermediate peptidase (MIP) that cleaves the COX4 precursor protein. This cobalt-induced MIP inhibition was however not observed in other human mitochondrial precursor sequences as previously predicted from comparison between human and yeast mitochondrial precursor sequences. | |abstract=Cobalt is often used as a hypoxia mimic in cell culture, because it stabilizes the α subunits of the transcription factor, HIF (hypoxia-inducible factor). We have previously shown that HIF stabilization due to a deficiency of the von Hippel Lindau protein (pVHL) in clear cell renal carcinoma (CRCC) was correlated to a down-regulation of oxidative phosphorylation. To better understand this mechanism, we have used CoCl2 in CRCC expressing stably transfected ''vhl''. We show that, in addition to its effect on HIF-α subunits, CoCl2 prevented the normal processing of the precursor of cytochrome ''c'' oxidase (COX) subunit 4 and induced COX degradation very likely by inhibiting the mitochondrial intermediate peptidase (MIP) that cleaves the COX4 precursor protein. This cobalt-induced MIP inhibition was however not observed in other human mitochondrial precursor sequences as previously predicted from comparison between human and yeast mitochondrial precursor sequences. | ||
|keywords=Hypoxia-inducible factor | |keywords=Hypoxia-inducible factor, Cytochrome ''c'' oxidase subunit 4, Cytochrome ''c'' oxidase biogenesis, Mitochondrial precursor processing, Mitochondrial intermediate peptidase, Cobalt chloride, ''Homo sapiens'', ''Saccharomyces cerevisiae'' | ||
|mipnetlab=CZ Prague Houstek J | |||
|discipline=Biomedicine | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Biogenesis;mt-density, Genetic knockout;overexpression | |||
|diseases=Cancer | |||
|organism=Human, Saccharomyces cerevisiae, Fungi | |||
|preparations=Intact cells | |||
|enzymes=Complex IV;cytochrome c oxidase | |||
|topics=Inhibitor | |||
|couplingstates=ROUTINE | |||
|instruments=Oxygraph-2k | |||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} | ||
Latest revision as of 14:30, 26 March 2018
| Hervouet E, Pecina P, Demont J, Vojtíšková A, Simonnet H, Houštek J, Godinot C (2006) Inhibition of cytochrome c oxidase subunit 4 precursor processing by the hypoxia mimic cobalt chloride. Biochem Biophys Res Commun 344:1086-93. |
Hervouet E, Pecina P, Demont J, Vojtiskova A, Simonnet H, Houstek J, Godinot C (2006) Biochem Biophys Res Commun
Abstract: Cobalt is often used as a hypoxia mimic in cell culture, because it stabilizes the α subunits of the transcription factor, HIF (hypoxia-inducible factor). We have previously shown that HIF stabilization due to a deficiency of the von Hippel Lindau protein (pVHL) in clear cell renal carcinoma (CRCC) was correlated to a down-regulation of oxidative phosphorylation. To better understand this mechanism, we have used CoCl2 in CRCC expressing stably transfected vhl. We show that, in addition to its effect on HIF-α subunits, CoCl2 prevented the normal processing of the precursor of cytochrome c oxidase (COX) subunit 4 and induced COX degradation very likely by inhibiting the mitochondrial intermediate peptidase (MIP) that cleaves the COX4 precursor protein. This cobalt-induced MIP inhibition was however not observed in other human mitochondrial precursor sequences as previously predicted from comparison between human and yeast mitochondrial precursor sequences. • Keywords: Hypoxia-inducible factor, Cytochrome c oxidase subunit 4, Cytochrome c oxidase biogenesis, Mitochondrial precursor processing, Mitochondrial intermediate peptidase, Cobalt chloride, Homo sapiens, Saccharomyces cerevisiae
• O2k-Network Lab: CZ Prague Houstek J
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Genetic knockout;overexpression
Pathology: Cancer
Organism: Human, Saccharomyces cerevisiae, Fungi
Preparation: Intact cells Enzyme: Complex IV;cytochrome c oxidase Regulation: Inhibitor Coupling state: ROUTINE
HRR: Oxygraph-2k
