Difference between revisions of "Krumschnabel 2016a Abstract MitoFit Science Camp 2016"
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{{Abstract | {{Abstract | ||
|title=O2k-Protocols: Mitochondrial preparations for HRR | |title=O2k-Protocols: Mitochondrial preparations for HRR. | ||
|info=[http://wiki.oroboros.at/index.php/MiPNet21.13_Comparison_mt-preparations MiPNet21.13] | |||
|authors=Doerrier C, Eigentler A, Krautschneider M, Schoepf B, Fontana-Ayoub M, Krumschnabel G, Gnaiger E | |authors=Doerrier C, Eigentler A, Krautschneider M, Schoepf B, Fontana-Ayoub M, Krumschnabel G, Gnaiger E | ||
|year=2016 | |year=2016 | ||
|event=MitoFit Science Camp 2016 Kuehtai AT | |event=MitoFit Science Camp 2016 Kuehtai AT | ||
|abstract=Mitochondrial (mt) preparations typically applied in high-resolution respirometry (HRR) comprise isolated mitochondria (Imt), tissue homogenates (Thom), and permeabilized tissue fibres (Pfi), each of which has its pros and cons. Here, different mt preparations from two mammalian experimental model systems, mouse and beef heart, are evaluated and compared regarding their suitability in HRR. It is shown that different mt preparations may result in almost identical (mouse) or at least comparable (beef) mitochondrial coupling control and respiratory capacities. Further, we show that homogenates produced with the PBI-Shredder provide high quality mt preparations, which may be quickly prepared with very little sample required. | |abstract=Mitochondrial (mt) preparations typically applied in high-resolution respirometry (HRR) comprise isolated mitochondria (Imt), tissue homogenates (Thom), and permeabilized tissue fibres (Pfi), each of which has its pros and cons. Here, different mt preparations from two mammalian experimental model systems, mouse and beef heart, are evaluated and compared regarding their suitability in HRR. It is shown that different mt preparations may result in almost identical (mouse) or at least comparable (beef) mitochondrial coupling control and respiratory capacities. Further, we show that homogenates produced with the PBI-Shredder provide high quality mt preparations, which may be quickly prepared with very little sample required. | ||
|mipnetlab=AT Innsbruck | |mipnetlab=AT Innsbruck Oroboros | ||
}} | }} | ||
== Affiliations == | |||
1-Oroboros Instruments, Innsbruck; 2-D. Swarovski Research Lab, Dept Visceral, Transplant and Thoracic Surgery, Innsbruck Med Univ, Austria. | |||
{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP | |area=Respiration, Instruments;methods, Comparative MiP;environmental MiP | ||
|organism=Mouse | |organism=Mouse | ||
|tissues=Heart | |tissues=Heart | ||
|preparations=Homogenate | |preparations=Permeabilized tissue, Homogenate, Isolated mitochondria | ||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=N, S, NS, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|event=D1 | |||
|additional=MitoFit Science Camp 2016 | |additional=MitoFit Science Camp 2016 | ||
}} | }} | ||
Latest revision as of 18:41, 10 January 2022
| O2k-Protocols: Mitochondrial preparations for HRR. |
Link: MiPNet21.13
Doerrier C, Eigentler A, Krautschneider M, Schoepf B, Fontana-Ayoub M, Krumschnabel G, Gnaiger E (2016)
Event: MitoFit Science Camp 2016 Kuehtai AT
Mitochondrial (mt) preparations typically applied in high-resolution respirometry (HRR) comprise isolated mitochondria (Imt), tissue homogenates (Thom), and permeabilized tissue fibres (Pfi), each of which has its pros and cons. Here, different mt preparations from two mammalian experimental model systems, mouse and beef heart, are evaluated and compared regarding their suitability in HRR. It is shown that different mt preparations may result in almost identical (mouse) or at least comparable (beef) mitochondrial coupling control and respiratory capacities. Further, we show that homogenates produced with the PBI-Shredder provide high quality mt preparations, which may be quickly prepared with very little sample required.
• O2k-Network Lab: AT Innsbruck Oroboros
Affiliations
1-Oroboros Instruments, Innsbruck; 2-D. Swarovski Research Lab, Dept Visceral, Transplant and Thoracic Surgery, Innsbruck Med Univ, Austria.
Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP
Organism: Mouse
Tissue;cell: Heart
Preparation: Permeabilized tissue, Homogenate, Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
Event: D1
MitoFit Science Camp 2016
