Meszaros 2024 Transpl Int
| Meszaros AT, Weissenbacher A, Schartner M, Egelseer-Bruendl T, Hermann M, Unterweger J, Mittelberger C, Reyer BA, Hofmann J, Zelger BG, Hautz T, Resch T, Margreiter C, Maglione M, Komlódi T, Ulmer H, Cardini B, Troppmair J, Öfner D, Gnaiger E, Schneeberger S, Oberhuber R (2024) The predictive value of graft viability and bioenergetics testing towards the outcome in liver transplantation. Transpl Int 37. https://doi.org/10.3389/ti.2024.12380. |
Meszaros AT, Weissenbacher A, Schartner Melanie, Egelseer-Bruendl T, Hermann M, Unterweger J, Mittelberger C, Reyer BA, Hofmann Julia, Zelger BG, Hautz T, Resch T, Margreiter C, Maglione M, Komlodi Timea, Ulmer H, Cardini B, Troppmair J, Oefner D, Gnaiger Erich, Schneeberger Stefan, Oberhuber R (2024) Transpl Int
Abstract: Donor organ biomarkers with sufficient predictive value in liver transplantation (LT) are lacking. We herein evaluate liver viability and mitochondrial bioenergetics for their predictive capacity towards the outcome in LT. We enrolled 43 consecutive patients undergoing LT. Liver biopsy samples taken upon arrival after static cold storage were assessed by histology, real-time confocal imaging analysis (RTCA), and high-resolution respirometry (HRR) for mitochondrial respiration of tissue homogenates. Early allograft dysfunction (EAD) served as primary endpoint. HRR data were analysed with a focus on the efficacy of ATP production or P-L control efficiency, calculated as 1-L/P from the capacity of oxidative phosphorylation P and non-phosphorylating respiration L. Twenty-two recipients experienced EAD. Pre-transplant histology was not predictive of EAD. The mean RTCA score was significantly lower in the EAD cohort (−0.75 ± 2.27) compared to the IF cohort (0.70 ± 2.08; p = 0.01), indicating decreased cell viability. P-L control efficiency was predictive of EAD (0.76 ± 0.06 in IF vs. 0.70 ± 0.08 in EAD-livers; p = 0.02) and correlated with the RTCA score. Both RTCA and P-L control efficiency in biopsy samples taken during cold storage have predictive capacity towards the outcome in LT. Therefore, RTCA and HRR should be considered for risk stratification, viability assessment, and bioenergetic testing in liver transplantation.
• Bioblast editor: Gnaiger E • O2k-Network Lab: AT Innsbruck Schneeberger S, AT Innsbruck Oroboros
Labels: MiParea: Respiration, mt-Medicine, Patients
Pathology: Other
Stress:Ischemia-reperfusion
Organism: Human
Tissue;cell: Liver
Preparation: Homogenate
Regulation: Coupling efficiency;uncoupling Coupling state: LEAK, OXPHOS Pathway: N, S, NS HRR: Oxygraph-2k
