MiPNet10.04 CellRespiration: Difference between revisions

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Garedew A, Haffner B, Huetter E, Gnaiger E (2005) An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04.

» Open Access-MiPNet10.04.pdf; Versions

OROBOROS (2005) Mitochondr Physiol Network

Abstract: O2k-Protocol for Phosphorylation Control in Cell Respiration

Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; Schröcken, Austria).

>> O2k-Protocols:Overall contents

>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_OROBOROS

Labels: MiParea: Respiration, Instruments;methods

Organism: Mouse Tissue;cell: Blood cells Preparation: Intact cells

Regulation: Coupling efficiency;uncoupling, Uncoupler Coupling state: LEAK, ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Oxygraph-2k, TIP2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

O2k-Demo

Further information

>> MiPNet10.04

Version 6: 2012-04-07
Version 1: 2005

References

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 {{Publication
 |title=Garedew A, Haffner B, Huetter E, Gnaiger E (2005) An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04.
-|info=[[Media:MiPNet10.04 CellRespiration.pdf|'''Open Access-MiPNet10.04''']]; [http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]
+|info=[[Media:MiPNet10.04 CellRespiration.pdf|'''Open Access-MiPNet10.04.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet10.04_CellRespiration.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]
 |authors=OROBOROS
 |year=2005