Difference between revisions of "MiPNet12.11 MitoRespiration"
| Line 9: | Line 9: | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|ecophysiological_model_organism=Artemia | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |taxonomic group=Crustaceans | ||
| | |ecomodel=Artemia | ||
|preparations=Isolated Mitochondria | |preparations=Isolated Mitochondria | ||
|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
| Line 20: | Line 21: | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|ecophysiological_model_organism=Artemia | |||
}} | }} | ||
*[[Gnaiger 2012 MitoPathways References|References]] | *[[Gnaiger 2012 MitoPathways References|References]] | ||
Revision as of 16:22, 8 August 2013
| Hand SC, Lemieux H, Gnaiger E. Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. |
Hand SC, Lemieux H, Gnaiger E (
Abstract: Methodological and conceptual features of high-resolution respirometry are illustrated in an experiment with isolated mitochondria in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations applied to study respiratory control ratios and respiratory capacity in isolated mitochondria. Application of the DatLab 4.2 software is shown for on-line data analysis. The following guideline describes the experimental protocol and includes a short discussion of results. The experiments were carried out by participants of an O2k-Course on HRR in December 2006 (IOC36; Schröcken, Austria).
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
