Difference between revisions of "MiPNet12.11 MitoRespiration"
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Hand SC, Lemieux H, Gnaiger E. Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. | |title=Hand SC, Lemieux H, Gnaiger E. Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. | ||
|info=[http://www.oroboros.at/ | |info=[http://www.oroboros.at/?ioc36-demo MiPNet12.11] | ||
|authors=Hand SC, Lemieux H, Gnaiger E | |authors=Hand SC, Lemieux H, Gnaiger E | ||
|year=* | |year=* | ||
Line 12: | Line 12: | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Instruments and methods | |||
|taxonomic group=Crustaceans | |taxonomic group=Crustaceans | ||
|ecomodel=Artemia | |ecomodel=Artemia | ||
Line 17: | Line 18: | ||
|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CII | |substratestates=CII | ||
|instruments=Oxygraph-2k, Protocol | |instruments=Oxygraph-2k, Protocol | ||
|additional=O2k-Demo | |additional=O2k-Demo | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication |
Revision as of 12:07, 10 August 2013
Hand SC, Lemieux H, Gnaiger E. Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. |
ยป MiPNet12.11
Hand SC, Lemieux H, Gnaiger E (
Abstract: Methodological and conceptual features of high-resolution respirometry are illustrated in an experiment with isolated mitochondria in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations applied to study respiratory control ratios and respiratory capacity in isolated mitochondria. Application of the DatLab 4.2 software is shown for on-line data analysis. The following guideline describes the experimental protocol and includes a short discussion of results. The experiments were carried out by participants of an O2k-Course on HRR in December 2006 (IOC36; Schrรถcken, Austria).
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Respiration, Instruments and methods"Instruments and methods" is not in the list (Respiration, Instruments;methods, mt-Biogenesis;mt-density, mt-Structure;fission;fusion, mt-Membrane, mtDNA;mt-genetics, nDNA;cell genetics, Genetic knockout;overexpression, Comparative MiP;environmental MiP, Gender, ...) of allowed values for the "MiP area" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo