Difference between revisions of "MiPNet12.11 MitoRespiration"
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{{Publication | {{Publication | ||
|title=Hand SC, Lemieux H, Gnaiger E | |title=Hand SC, Lemieux H, Gnaiger E (2007) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. | ||
|info=[http://www.oroboros.at/?IOC36-demo MiPNet12.11] | |info=[http://www.oroboros.at/?IOC36-demo MiPNet12.11] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year= | |year=2007 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=Methodological and conceptual features of high-resolution respirometry are illustrated in an experiment with isolated mitochondria in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations applied to study respiratory control ratios and respiratory capacity in isolated mitochondria. Application of the DatLab 4.2 software is shown for on-line data analysis. The following guideline describes the experimental protocol and includes a short discussion of results. The experiments were carried out by participants of an O2k-Course on HRR in December 2006 (IOC36; Schröcken, Austria). | |abstract=Methodological and conceptual features of high-resolution respirometry are illustrated in an experiment with isolated mitochondria in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations applied to study respiratory control ratios and respiratory capacity in isolated mitochondria. Application of the DatLab 4.2 software is shown for on-line data analysis. The following guideline describes the experimental protocol and includes a short discussion of results. The experiments were carried out by participants of an O2k-Course on HRR in December 2006 (IOC36; Schröcken, Austria). | ||
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|ecophysiological_model_organism=Artemia | |ecophysiological_model_organism=Artemia | ||
}} | }} | ||
* Version 5: 2011-12-11 | |||
* Version 1: 2007 | |||
*[[Gnaiger 2012 MitoPathways References|References]] | *[[Gnaiger 2012 MitoPathways References|References]] | ||
[[Category:OroboPedia]] | |||
Revision as of 09:22, 25 August 2013
| Hand SC, Lemieux H, Gnaiger E (2007) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. |
OROBOROS (2007) Mitochondr Physiol Network
Abstract: Methodological and conceptual features of high-resolution respirometry are illustrated in an experiment with isolated mitochondria in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations applied to study respiratory control ratios and respiratory capacity in isolated mitochondria. Application of the DatLab 4.2 software is shown for on-line data analysis. The following guideline describes the experimental protocol and includes a short discussion of results. The experiments were carried out by participants of an O2k-Course on HRR in December 2006 (IOC36; Schröcken, Austria).
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Artemia
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Coupling efficiency;uncoupling Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
- Version 5: 2011-12-11
- Version 1: 2007
