Difference between revisions of "MiPNet12.11 MitoRespiration"
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{{Publication | {{Publication | ||
|title=Hand SC, Lemieux H, Gnaiger E (2007) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. | |title=Hand SC, Lemieux H, Gnaiger E (2007) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. | ||
|info='''Open Access - [ | |info=[[Media:MiPNet12.11 MitoRespiration.pdf|'''Open Access-MiPNet12.11.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet12.11_MitoRespiration.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year=2007 | |year=2007 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=Methodological and conceptual features of high-resolution respirometry are illustrated in an experiment with isolated mitochondria in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations applied to study respiratory control ratios and respiratory capacity in isolated mitochondria. Application of the DatLab 4.2 software is shown for on-line data analysis. The following guideline describes the experimental protocol and includes a short discussion of results. The experiments were carried out by participants of an O2k-Course on HRR in December 2006 (IOC36; Schröcken, Austria). | |abstract='''O2k-Protocols for Basic Protocol with Isolated Mitochondria: LEAK, OXPHOS, ETS, ROX.''' | ||
Methodological and conceptual features of high-resolution respirometry are illustrated in an experiment with isolated mitochondria in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations applied to study respiratory control ratios and respiratory capacity in isolated mitochondria. Application of the DatLab 4.2 software is shown for on-line data analysis. The following guideline describes the experimental protocol and includes a short discussion of results. The experiments were carried out by participants of an O2k-Course on HRR in December 2006 (IOC36; Schröcken, Austria). | |||
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | |||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | |||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
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|ecophysiological_model_organism=Artemia | |ecophysiological_model_organism=Artemia | ||
}} | }} | ||
== Further information == | |||
:>> [http://www.oroboros.at/?IOC36-demo MiPNet12.11] | |||
* Version 5: 2011-12-11 | * Version 5: 2011-12-11 | ||
* Version 1: 2007 | * Version 1: 2007 | ||
Revision as of 08:24, 18 October 2013
| Hand SC, Lemieux H, Gnaiger E (2007) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11. |
» Open Access-MiPNet12.11.pdf; Versions
OROBOROS (2007) Mitochondr Physiol Network
Abstract: O2k-Protocols for Basic Protocol with Isolated Mitochondria: LEAK, OXPHOS, ETS, ROX.
Methodological and conceptual features of high-resolution respirometry are illustrated in an experiment with isolated mitochondria in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations applied to study respiratory control ratios and respiratory capacity in isolated mitochondria. Application of the DatLab 4.2 software is shown for on-line data analysis. The following guideline describes the experimental protocol and includes a short discussion of results. The experiments were carried out by participants of an O2k-Course on HRR in December 2006 (IOC36; Schröcken, Austria).
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Artemia
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Coupling efficiency;uncoupling Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
Further information
- >> MiPNet12.11
- Version 5: 2011-12-11
- Version 1: 2007
