Difference between revisions of "MiPNet15.03 O2k-MultiSensor-ISE"
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'''O2k-Manual''': The ion selective electrode (ISE) is a modular O2k-MultiSensor extension of the Oroboros O2k. ISE yield a potentiometric (voltage) signal simultaneously with the oxygen signal in both chambers of the O2k. The ISE system consists of separate reference and measuring electrodes selective for hydrophobic cations (TPP<sup>+</sup>, TPMP<sup>+</sup>), Ca<sup>2+</sup>, Mg<sup>2+</sup>, etc. This O2k-Manual describes the handling and application of the ISE system. | '''O2k-Manual''': The ion selective electrode (ISE) is a modular O2k-MultiSensor extension of the Oroboros O2k. ISE yield a potentiometric (voltage) signal simultaneously with the oxygen signal in both chambers of the O2k. The ISE system consists of separate reference and measuring electrodes selective for hydrophobic cations (TPP<sup>+</sup>, TPMP<sup>+</sup>), Ca<sup>2+</sup>, Mg<sup>2+</sup>, etc. This O2k-Manual describes the handling and application of the ISE system. | ||
:» Product: [[O2k-TPP+ ISE-Module]], [[Oroboros O2k-Catalogue]] | :» Product: [[O2k-TPP+ ISE-Module]], [[Oroboros O2k-Catalogue]] | ||
|keywords=[[HRR]], [[O2k-MultiSensor]] | |keywords=[[HRR]], [[O2k-MultiSensor]] | ||
|mipnetlab=AT_Innsbruck_Oroboros | |mipnetlab=AT_Innsbruck_Oroboros | ||
}} | }} | ||
{{Technical support integrated}} | {{Technical support integrated}} | ||
::::» ::::» [[Tetraphenylphosphonium#O2k-technical_support |TPP: O2k-technical_support mt-membrane potential]] | |||
::::» [[ | |||
::::» [[Calcium#O2k-technical_support |Calcium: O2k-technical_support]] | ::::» [[Calcium#O2k-technical_support |Calcium: O2k-technical_support]] | ||
::::» Other ions beside TPP+ TPMP+, and Ca2+: There are ion selective membranes available, e.g. from Sigma Aldrich, for a wide variety of species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods. | ::::» Other ions beside TPP+ TPMP+, and Ca2+: There are ion selective membranes available, e.g. from Sigma Aldrich, for a wide variety of species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods. |
Revision as of 19:09, 8 September 2018
MiPNet15.03 O2k-MultiSensor-ISE
O2k-MultiSensor system with ion selective electrodes (ISE). |
» »Versions
Oroboros (2014-03-06) Mitochondr Physiol Network
Abstract: Fasching M, Gnaiger E (2014) O2k-MultiSensor system with ion selective electrodes (ISE). Mitochondr Physiol Network 15.03(05):1-20. -
O2k-Manual: The ion selective electrode (ISE) is a modular O2k-MultiSensor extension of the Oroboros O2k. ISE yield a potentiometric (voltage) signal simultaneously with the oxygen signal in both chambers of the O2k. The ISE system consists of separate reference and measuring electrodes selective for hydrophobic cations (TPP+, TPMP+), Ca2+, Mg2+, etc. This O2k-Manual describes the handling and application of the ISE system.
- » Product: O2k-TPP+ ISE-Module, Oroboros O2k-Catalogue
• Keywords: HRR, O2k-MultiSensor
• O2k-Network Lab: AT_Innsbruck_Oroboros
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- » ::::» TPP: O2k-technical_support mt-membrane potential
- » Calcium: O2k-technical_support
- » Other ions beside TPP+ TPMP+, and Ca2+: There are ion selective membranes available, e.g. from Sigma Aldrich, for a wide variety of species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods.
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, TPP, O2k-Manual, O2k-Protocol
O2k-MultiSensor, DatLab, DL6, DL7, DL6a7