MiPNet15.09. Yeast: HRR Reference Assay

Simonovik B, Gnaiger E (2010) A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. Mitochondr Physiol Network 15.09.

» File:MiPNet15.09 mtRA-Yeast.pdf -Open Access

OROBOROS (2010) Mitochondr Physiol Network

Abstract: A mitochondrial reference assay (mtRA) was designed for high-resolution respirometry, to evaluate the reproducibility of cell respiration with baker’s yeast (Saccharomyces cerevisiae). Quality control is an integral part of quality assurance (QA), which is becoming increasingly important in high-resolution respirometry for basic and clinical research, as well as industrial applications. Quality control will ensure comparability of quantitative results obtained by different research groups. • Keywords: Yeast: HRR Reference Assay

• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS

Labels: MiParea: Respiration, Instruments;methods 

Organism: Saccharomyces cerevisiae 

Coupling state: ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Demo Experiment 

• Version 1: 2010
• Version 2: 2012-01-05