Difference between revisions of "MiPNet15.09. Yeast: HRR Reference Assay"

Revision as of 13:25, 18 October 2013

Simonovik B, Gnaiger E (2010) A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. Mitochondr Physiol Network 15.09.

» Open Access-MiPNet15.09.pdf;Versions

OROBOROS (2010) Mitochondr Physiol Network

Abstract: O2k-Protocol for High-Resolution Respirometry Using Freeze-Dried Baker’s Yeast.

A mitochondrial reference assay (mtRA) was designed for high-resolution respirometry, to evaluate the reproducibility of cell respiration with baker’s yeast (Saccharomyces cerevisiae). Quality control is an integral part of quality assurance (QA), which is becoming increasingly important in high-resolution respirometry for basic and clinical research, as well as industrial applications. Quality control will ensure comparability of quantitative results obtained by different research groups.

>> O2k-Protocols:Overall contents

>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue

• Keywords: Yeast: HRR Reference Assay

• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS

Labels: MiParea: Respiration, Instruments;methods

Organism: Saccharomyces cerevisiae

Coupling state: ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

Demo Experiment

Further information

Version 1: 2010
Version 2: 2012-01-05

@@ Line 1: / Line 1: @@
 {{Publication
 |title=Simonovik B, Gnaiger E (2010) A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. Mitochondr Physiol Network 15.09.
-|info='''Bioblast Access - [[File:MiPNet15.09 mtRA-Yeast.pdf]]'''
+|info=[[Media:MiPNet15.09 mtRA-Yeast.pdf|'''Open Access-MiPNet15.09.pdf''']];[http://www.bioblast.at/index.php/File:MiPNet15.09_mtRA-Yeast.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]
 |authors=OROBOROS
 |year=2010
 |journal=Mitochondr Physiol Network
-|abstract=A mitochondrial reference assay (mtRA) was designed for high-resolution respirometry, to evaluate the reproducibility of cell respiration with baker’s yeast (Saccharomyces cerevisiae). Quality control is an integral part of quality assurance (QA), which is becoming increasingly important in high-resolution respirometry for basic and clinical research, as well as industrial applications. Quality control will ensure comparability of quantitative results obtained by different research groups.
+|abstract='''O2k-Protocol for High-Resolution Respirometry Using Freeze-Dried Baker’s Yeast.'''
+A mitochondrial reference assay (mtRA) was designed for high-resolution respirometry, to evaluate the reproducibility of cell respiration with baker’s yeast (Saccharomyces cerevisiae). Quality control is an integral part of quality assurance (QA), which is becoming increasingly important in high-resolution respirometry for basic and clinical research, as well as industrial applications. Quality control will ensure comparability of quantitative results obtained by different research groups.
+:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]
+:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
 |keywords=Yeast: HRR Reference Assay
 |mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS
@@ Line 22: / Line 27: @@
 |articletype=Protocol; Manual, MiPNet-online Publication
 }}
+== Further information ==
 * Version 1: 2010
 * Version 2: 2012-01-05