Difference between revisions of "MiPNet15.09. Yeast: HRR Reference Assay"
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:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | :>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
|keywords=Yeast: HRR Reference Assay | |keywords=Yeast: HRR Reference Assay | ||
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS | |mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS | ||
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|substratestates=ROX | |substratestates=ROX | ||
|instruments=Oxygraph-2k, Protocol | |instruments=Oxygraph-2k, Protocol | ||
|additional=Demo | |additional=O2k-Demo | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
}} | }} | ||
== Further information == | == Further information == | ||
* Version 1: 2010 | * Version 1: 2010 |
Revision as of 19:02, 18 October 2013
Simonovik B, Gnaiger E (2010) A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. Mitochondr Physiol Network 15.09. |
» Open Access-MiPNet15.09.pdf; Versions
OROBOROS (2010) Mitochondr Physiol Network
Abstract: O2k-Protocol for High-Resolution Respirometry Using Freeze-Dried Baker’s Yeast.
A mitochondrial reference assay (mtRA) was designed for high-resolution respirometry, to evaluate the reproducibility of cell respiration with baker’s yeast (Saccharomyces cerevisiae). Quality control is an integral part of quality assurance (QA), which is becoming increasingly important in high-resolution respirometry for basic and clinical research, as well as industrial applications. Quality control will ensure comparability of quantitative results obtained by different research groups.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• Keywords: Yeast: HRR Reference Assay
• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Saccharomyces cerevisiae
Coupling state: ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
Further information
- Version 1: 2010
- Version 2: 2012-01-05