MiPNet19.05 IOC90 Bolzano IT: Difference between revisions
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'''16:00 β 16.45Β Β Β Β Round table discussion: Experience with mitochondrial respiration taking the Oxygraph-2k to >4,000 m''' | '''16:00 β 16.45Β Β Β Β Round table discussion: Experience with mitochondrial respiration taking the Oxygraph-2k to >4,000 m''' | ||
: [[Image:Erich Gnaiger Wolstenhjolm Fjord March 2004.jpg|90px|link=http://wiki.oroboros.at/index.php/Gnaiger_Erich|Erich Gnaiger, Greenland]] [[Image:Boushel Robert C.JPG|100px|link=http://wiki.oroboros.at/index.php/Boushel_Robert_C|Robert Boushel]] [[Image:Chicco Headshot.jpg|75px|link=http://wiki.oroboros.at/index.php/Chicco_Adam_J|Adam Chicco]] [[Image:Murray Andrew J.jpg|130px|link=http://wiki.oroboros.at/index.php/Murray_Andrew_J|Andrew Murray]] [[Image: | : [[Image:Erich Gnaiger Wolstenhjolm Fjord March 2004.jpg|90px|link=http://wiki.oroboros.at/index.php/Gnaiger_Erich|Erich Gnaiger, Greenland]] [[Image:Boushel Robert C.JPG|100px|link=http://wiki.oroboros.at/index.php/Boushel_Robert_C|Robert Boushel]] [[Image:Chicco Headshot.jpg|75px|link=http://wiki.oroboros.at/index.php/Chicco_Adam_J|Adam Chicco]] [[Image:Murray Andrew J.jpg|130px|link=http://wiki.oroboros.at/index.php/Murray_Andrew_J|Andrew Murray]] [[Image:Horscroft James A.jpg|85px|link=http://wiki.oroboros.at/index.php/Horscroft_James_A|James Horscroft]] | ||
Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function. | Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function. | ||
* [[Gnaiger Erich]], AT - [[Boushel Robert C]], SE - [[Chicco Adam J]], US - [[Murray Andrew J]], UK - [[Horscroft JA]], UK | * [[Gnaiger Erich]], AT - [[Boushel Robert C]], SE - [[Chicco Adam J]], US - [[Murray Andrew J]], UK - [[Horscroft JA]], UK |
Revision as of 09:28, 22 May 2014
Bolzano IT, 2014 May 27. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC90 at ISMM2014. |
Β» O2k-Workshop
OROBOROS (2014-05-27) Mitochondr Physiol Network
Abstract:
90th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry.
Info and preliminary programme: MiPNet19.05.pdf
β’ O2k-Network Lab: AT Innsbruck OROBOROS
Labels:
Stress:Hypoxia, Temperature
HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
ORO, IOC, 2014, Next
Conference
- ISMM2014 World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31
O2k-Workshop
- TUESDAY 2014-05-27 / 14.00 - 17.30
- Seminar room 8
The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H2O2 production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on βClinical Hypothermia and Mitochondrial Functionβ.
- More details? Consult Β»Hypothermia-Network: Mitochondria in the Cold.
Introduction
14:00 β 14:45 Introduction: high-resolution respirometry and mt-function in extreme environments
Gnaiger Erich, CEO;
Fontana-Ayoub Mona; Biomedical assistant;
Fleischmann Sandra; Administrative assistant
- OROBOROS INSTRUMENTS - General introduction into the Oxygraph-2k and high-resolution respirometry, O2k-Demo experiment on mitochondrial respiratory function under hypoxic steady-state (O2k with module Titration-Injection microPump).
Demo experiment
14:45 β 15:30 Demo experiment: mitochondrial respiration and H2O2 production under hypoxia
15:30 β 16:00 Coffee break
Round table: Hypoxia and mitochondrial function - practical experience
16:00 β 16.45 Round table discussion: Experience with mitochondrial respiration taking the Oxygraph-2k to >4,000 m
Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function.
- Gnaiger Erich, AT - Boushel Robert C, SE - Chicco Adam J, US - Murray Andrew J, UK - Horscroft JA, UK
Round table: Hypothermia and mitochondrial function - perspectives
16:45 β 17:00 Round table discussion: Clinical Hypothermia and Mitochondrial Function
Keynote statements by
- Strapazzon Giacomo, IT - Malacrida Sandro, IT - and ad hoc participants
Registration
- Visit us at the OROBOROS INSTRUMENTS exhibition booth from Monday to Wednesday, May 26 to 28.
- Here you can register for the OROBOROS O2k-Workshop until Tuesday morning, May 27. Free registration for ISMM participants.
Recommended reading
O2k-Core Manual
SUIT protocols for high-resolution respirometry
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. Β»Bioblast Access
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. Β»Bioblast Access
HRR and O2k-Fluorometry
- Β»Manual: O2k-Fluorescence LED2-Module
- Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. Β»Open Access
- Β»O2k-Fluorometry
Mitochondrial pathways
Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. Β»Open Access - handout to O2k-Workshop participants.
O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom.