MiPNet22.11 O2k-FluoRespirometer manual: Difference between revisions
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::::# Volume calibration of 2-mL O2k-chamber: Fill open chamber with 2.07 mL water (not 2.085 or 2.10 mL). The volume of the stopper capillary is 0.065 mL (not 0.085 mL). | ::::# Volume calibration of 2-mL O2k-chamber: Fill open chamber with 2.07 mL water (not 2.085 or 2.10 mL). The volume of the stopper capillary is 0.065 mL (not 0.085 mL). | ||
== Troubleshooting == | |||
::: ''-See'': [[Defective fluorescence module]] | |||
== Keywords == | == Keywords == |
Revision as of 15:53, 16 March 2021
MiPNet22.11 O2k-FluoRespirometer manual |
O2k-FluoRespirometer manual. |
Β» Β» Versions
Oroboros (2020-11-18) Mitochondr Physiol Network
Abstract: Gnaiger E, MΓ©szΓ‘ros A, Gradl L, Gradl P (2020) O2k-FluoRespirometer manual. Mitochondr Physiol Network 22.11(08):1-17.
- Β» Current O2k-series: NextGen-O2k Series XB and O2k Series J
- Β» Current software versions DatLab 8.0: MitoPedia: DatLab
- Further details: Β» MitoPedia: O2k-Open Support
- Β» Product: DatLab 7, O2k-FluoRespirometer, O2k-Catalogue
β’ O2k-Network Lab: AT_Innsbruck_Oroboros
Important updates
- Version 08 (2020-10-17) Section 5.4. O2k-chamber volume calibration
- Instructions are added for the 0.5-mL O2k-chamber volume calibration (O2k-sV-Module).
- Volume calibration of 2-mL O2k-chamber: Fill open chamber with 2.07 mL water (not 2.085 or 2.10 mL). The volume of the stopper capillary is 0.065 mL (not 0.085 mL).
Troubleshooting
Keywords
- Β»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Β» Volume
- Units: Liter [L]; 1 L = 1 dm3 = 10-3 m3; 1 mL = 1 cm3; 1 Β΅L = 1 mm3
- Β» Volume
- On chamber volume
- Components of the experimental O2k-chambers
- Β» Stirrer-Bar\white PVDF\15x6 mm
- Β» Stirrer-Bar sV\white PVDF\11.5x6.2 mm
- Β» Stopper\black PEEK\conical Shaft\central Port
- Β» Stopper sV\black PEEK\conical Shaft\central Port
- Β» O-ring\Viton\12.5x1 mm
- Β» O-ring sV\Viton\9.5x1 mm
- Β» Volume-Calibration Ring
- Β» Volume-Calibration Ring sV
- Β» Stopper-Spacer
- Β» Cover-Slip\black
- Β» O2k-Chamber Holder
- Β» O2k-Chamber Holder sV
- Β» OroboPOS-Holder
- Β» OroboPOS-Holder sV
- Maintencance
Labels: MiParea: Instruments;methods
HRR: O2k-Manual, Oxygraph-2k
O2k, DatLab, DL7, SeriesH