MiPNet23.03 IOC128 Cambridge UK
 Cambridge UK, 2018 May 7-8. Oroboros satellite O2k-Workshop on High-Resolution FluoRespirometry IOC128. |
Oroboros (2018-05-07) Mitochondr Physiol Network
Abstract:
Oroboros satellite O2k-Workshop on High-Resolution FluoRespirometry in cooperation with UK Cambridge Murray AJ. Cambridge, United Kingdom, 2018 May 7.
• O2k-Network Lab: AT_Innsbruck_OROBOROS, UK Cambridge Murray AJ
General information
- This O2k-Workshop takes place satellite to the Mitochondrial Medicine Conference in Hinxton, UK. Separate registration is required for this workshop.
Venue
- May 7
- The Graham Storey Room
- Trinity Hall - Cambridge
- Cambridge CB2 1TJ
- United Kingdom
- Venue
- May 8
- Department of Physiology, Development & Neuroscience
- University of Cambridge
Local organizers
Registration
- Download registration form: download pdf
- Limited number of participants.
Tutors and Lecturers
Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory
András Mészáros, MD., PhD., Chief Research Officer, Oroboros Instruments
Verena Laner, Chief operating officer (COO), Oroboros Instruments
Programme
- May 7 - full day: lectures and presentations
- May 8 - morning: lectures and presentations
- May 8 - afternoon: hands-on session
- Topics:
- Mitochondrial respiratory states and rates
- SUIT Protocols in DatLab 7
- Mitochondrial preparations: the versatility of O2k applications
- Topics:
- Normoxia – hyperoxia – hypoxia
- O2 flux and mt membrane potential (TPP+ potentiometry and safranin/TMRM fluorescence)
- O2 flux and H2O2 production: physiology and pathology
- Experimental demo for
- 1. Getting started with the O2k and DatLab 7
- 2. DatLab 7 innovations
- Experimental demo for
- Hands-on (2018-05-08)
- O2k calibration and quality control (O2 and H2O2)
- Cell respiration and H2O2 production
- Hands-on (2018-05-08)
Programme
Monday May 07
| Time | Topic | Materials | |
|---|---|---|---|
| 09:00-09:15 | Arrival tea and coffee | ||
| 09:15-10:15 |  |
Erich Gnaiger
Lecture: Mitochondrial respiratory states and rates |
|
| 10:15-11:00 | |
Erich Gnaiger
Lecture: Mitochondrial preparations: the versatility of O2k applications |
|
| 11:00-11:15 | Morning coffee break | ||
| 11:15-12:15 | 
|
Erich Gnaiger
Demo experiment: Getting started with the O2k and DatLab 7 |
|
| 12:15-13:00 |
|
Erich Gnaiger
Demo experiment: DatLab 7 innovations |
|
| 13:00-14:00 | Lunch break | ||
| 14:00-15:30 |
|
Erich Gnaiger
Lecture: Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control. Mitochondrial pathways and coupling. |
Doerrier_2018_Methods_Mol_Biol |
| 15:30-15:45 | Tea break | ||
| 15:45-17:00 | |
Erich Gnaiger
Lecture: Normoxia – hyperoxia – hypoxia. |
Tuesday May 08
| Time | Topic | Materials | |
|---|---|---|---|
| 09:00-09:15 | Arrival tea and coffee | ||
| 09:15-09:45 | |
Erich Gnaiger
Lecture: O2 flux and H2O2 production: physiology and pathology |
|
| 09:45-11:00 |
|
Hands-on: O2k calibration and quality control (O2 and H2O2) |
|
| 11:00-11:15 | Morning coffee break | ||
| 11:15-13:00 |
|
Hands-on: O2k-FluoRespirometry for measurement of cell respiration and H2O2 production |
|
| 13:00-14:00 | Lunch break | ||
| 14:00-15:30 |
|
Hands-on: Data analysis, interpretation and discussion. |
|
| 15:30-15:45 | Tea break | ||
| 15:45-17:00 | |
Erich Gnaiger
Lecture: O2 flux and mt membrane potential (TPP+ potentiometry and safranin/TMRM fluorescence). |
Preliminary list of participants
| Participant | Institution | |
|---|---|---|
| Davies Katie | University of Cambridge, UK | |
| Fornaro Mara | Novartis Institutes for Biomedical Research, CH | |
| Garrud Tessa | University of Cambridge, UK | |
| Kapoor Rishab | Vanderbilt University Medical Center, USA | |
|
Knapp-Wilson Amber | University of Bristol , UK |
| Krause Fynn Nidas | University of Cambridge, UK | |
| Martin Daniel | UK London Martin DS - University College London, UK | |
| Sowton Alice | UK Cambridge Murray AJ - Trinity Hall, UK | |
| Stanzani Giacomo | UK London Duchen MR - University College London, UK | |
| Stevens Jia Liu | UK London Martin DS - University College London, UK | |
|
Tong Wen | University of Cambridge, UK |
|
Yong Hannah | UK Cambridge Murray AJ - University of Cambridge, UK |
Mitochondrial Medicine Conference Hinxton
Contact
- Oroboros Instruments
- High-Resolution FluoRespirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Labels:
HRR: Oxygraph-2k
ORO, IOC, O2k-Network, 2018, Next
O2k-Workshops are listed as MitoGlobal Events.
