From Bioblast
Description
Proline, C5H9NO2, is an amino acid which occurs under physiological conditions mainly as the nonpolar form, Pro, with pKa1 = 1.99 pKa2 = 10.96. Proline is a substrate that supports both proline dehydrogenase pathway control state and the glutamate anaplerotic pathway control state. Proline is oxidized in the mitochondrial matrix by L-proline:quinone oxidoreductase (proline dehydrogenase, PDH) to delta-1-pyrroline-5-carboxylate. delta-1-pyrroline-5-carboxylate is converted to glutamate by 1-pyrroline-5-carboxylate dehydrogenase.
Abbreviation: Pro
MitoPedia topics: Substrate and metabolite
Application in HRR
- Preparation of 2 M stock solution (dissolved in H2O; M.W. = 115.13)
- Weigh 1.1513 g L-Proline and transfer to a 5 mL volumetric glass flask.
- Add to 5 mL of H2O and divide into 0.5 mL portions.
- Store at -20 Β°C.
- Β» O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 10 Β΅L using a 25 Β΅l syringe (2 mL O2k-Chamber).
- Final concentration: 10 mM.